Expressão diferencial de proteínas de amostras Campylobacter fetus subsp venerealis após passagens sucessivas em novilhas

• Ano de defesa: 2009
• Autor(a) principal: Ana Paula Reinato Stynen
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: proteomica; Campylobacters fetus venerealis; fatores de virulencia; bovino; Veterinária; Ciência Animal; Bovino Doenças; Proteínas; Campylobacter fetus
• Link de acesso: http://hdl.handle.net/1843/SMOC-9HCNTH

The bovine genital campylobacteriosis is a venereal disease, which leads to temporary infertility and sporadic abortion in cattle. So it has great importance in countries like Brazil, where large herds using natural service. In order to verify that the serial passage in heifers active proteins that are linked to the pathogenesis of infection and identify differentially expressed proteins in samples of Campylobacter fetus subsp venerealis, has been tested for adhesion and induction of transcription of interleukin-8 (IL-8) in infected epithelial cells and identification of proteins by mass spectrometry. It was found that serial passage of C. fetus subsp. venerealis heifers gradually leads to increased adhesion and induces transcription of IL-8 in HeLa cells. Serial passages of the microorganism "in vivo" lead to increased expression of proteins such as Chemotaxis Purine Binding (Chew), Hook Associated scourge, Lipoprotein and Possible Accepting chemotaxisis methyl, linked to the bacterial adherence, chemotaxis and motility important in bacterial colonization and the establishment infection.