Efeito do concentrado autólogo de plaquetas no tratamento da osteoartrose induzida em coelhos

• Ano de defesa: 2015
• Autor(a) principal: Fredy Esteban Osório Carmona
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: plasma rico em plaquetas; Osteoartrose; coelhos; Coelho Doenças Tratamento; Plaquetas (Sangue); Articulações Doenças
• Link de acesso: http://hdl.handle.net/1843/SMOC-A85GFW

The objectives of this study was to evaluate the effect of the autologous platelet concentrate (APC) on osteoarthritis (OA) induced in rabbits, through clinical, radiographic, ultrasonographic, laboratorial and arthroscopic evaluations, and to determinate the platelet derived growth factor (PDGF) and of transforming growth factor 1 (TGF-1) concentrations in plasma of rabbits treated with APC or Ringer Lactato. Were used 14 New Zealand rabbits with average body weight of 3,0 kg submitted to cranial cruciate ligament (LCCr) resection and substitution with fascia lata auto-graft guided by arthroscopy. The rabbits were distributed in two groups of seven animals each and received intra-articular injections of APC or Ringer Lactato. In the orthopedic evaluation the APC group showed significantly better results (p<0,05) than the control group rabbits in relation to the pain sensitivity, presence of articular effusion and member support and position in moment M3. It was observed significant statistically differences (p<0,05) in the flexion and extension angles in the right hindlimb between groups in the M3. It was not observed significant differences between the groups in the radiographic and ultrassonographic evaluation. It was observed between the groups significant statistically difference (p<0,05) in the synovial fluid evaluation just in relation to volume and protein parameters in the moment M3. It was verificated in the cellular counts, significant statistically diference (p<0,05) between the APC and the total blood in the moments M2 and M3. The medium plaquetary volume (MPV), however, was simillar. The PDGF concentration was statistically higer (p<0,05) in ACP group in the M3 in relation to the control group, but there was not statistically significant difference in the TGF-1 concentration between groups on any of the avaliated moments. The obtained result allow to conclude that the employed method to concentrate inactive platelets of rabbits was effective and that ACP have a favorable effect in joints affected by osteoarthritis (OA). The dosage of growth factors PDGF e TGF-1, can be used as a biological marker to evaluate the degenerative process