Efeito do estímulo vagal sobre a coagulação no modelo de choque hemorrágico controlado em ratos

• Ano de defesa: 2014
• Autor(a) principal: Roger Lage Alves
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: Ciências Aplicadas à Cirurgia e a Oftalmologia; Choque hemorrágico; Coagulação sanguínea; Modelos animais; Vagotomia; Sangue Distúrbios da coagulação; Ratos Wistar; Transtornos da coagulação sanguínea; Tromboelastografia/métodos; Citocinas
• Link de acesso: http://hdl.handle.net/1843/BUOS-9Q5GDS

Background_ In the present work the influence of vagal stimulation on the coagulation profile as demonstrated by tromboelastometria in a controlled hemorrhagic shock model was investigated. The trauma coagulopathy is associated to hemorrhagic shock and inflammation with increase of plasmatic IL-1, IL-6 and TNF levels. Cholinergic anti-inflammatory pathways were demonstrated to regulate inflammatory cytokines release. Vagal stimulation was proved to modify the inflammatory response pattern. The purpose of this paper was to investigate the role played by vagal stimulation on the hemorrhagic shock-induced coagulopathy. Methods _ Baseline and final measurements of thromboelastometry and cytokines were analyzed in rats submitted to hemorrhagic shock preceded or followed by cervical vagal stimulation ; data expressed as mean +/- SD were analyzed by ANOVA and Student t test. Results _ Hemorrhagic shock without vagal stimulation, worsens maximum clot firmness (MCF) and reduced IL-1 levels. Stimulation of the caudal end of the vagus nerve improved maximum clot firmness (MCF), clot formation time (CFT) and anticipated thrombin organization ( angle). Either bilateral vagotomy or bilateral stimulation of intact vagus before acute hemorrhagic shock did not modified the coagulation profile. Conclusions_ Caudal stimulation of the distal edge of a seccioned vagus improves coagulation and reduces IL-1 plasmatic levels after acute hypovolemic shock, Bilateral vagotomy or stimulation of intact vagus before hemorrhagic shock do not modify coagulation. Acute hypovolemic controlled hemorrhagic shock without vagal stimulation worsens maximum clot firmness.