Infecção helmíntica exerce efeito benéfico no desenvolvimento da obesidade experimental e suas consequências metabólicas

• Ano de defesa: 2017
• Autor(a) principal: Camila Pereira de Queiroz
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; Brasil; ICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA; Programa de Pós-Graduação em Bioquímica e Imunologia; UFMG
• Palavras-chave em Português: Heligmosomoides polygyrus; Inflamação; Imunorregulação; Nematospiroides dubius; Obesidade; Helmintíase
• Link de acesso: http://hdl.handle.net/1843/34520

Obesity is a worldwide disease defined by the excessive amount of fat that can cause damage to the health. The greatest concern about this disease is associated with the metabolic disorders developed with it, that can lead to metabolic syndrome. The helminth infection can induce various mechanisms capable of modulating and regulating the immune response of the host, ensuring their stay. These modulations, including the activation of T regulatory cells, end up being beneficial to the host, because it decreases the inflammation and impairs the development of inflammatory chronic diseases. Considering that, the present study aimed to verify the influence of the infection by Heligmosomoides polygyrus in the development of obesity and its consequences. Male C57BL/6, specific pathogen free, were fed a control diet or a high fat diet, for up to eight weeks, in the presence or not of the infection. Weight gain, caloric intake and egg counting in the stool were evaluated weekly. At the end of the experiment we also evaluated parameters related to insulin resistance, cholesterol, secretion of adipokines, inflammation and cellular migration to the adipose tissue. Despite no difference in the caloric intake between the animals fed with high fat diet, the infection was able to prevent the weight gain, also decreasing the weight of epididymal and subcutaneous fat. This effect does not occur due parasite spoliation because there was no difference between the groups that received control diet. Also, the infection prevented the development of adipocytes hypertrophy, insulin resistance and deregulation in the production of adipokines. The inflammation decreased, apparently in a manner independent of IL-10, and the percentage of T regulatory cells and eosinophils increased in the adipose tissue. Besides that, the infection was not able to reduce the oxidative stress, neither change the migration of inflammatory cells (Th17, ILC1, ILC3) to the adipose tissue. Overall, the infection prevented the exacerbated weight gain and the development of obesity associated consequences, probably by decreasing the inflammation, associated to the increase of T regulatory cells.