Desenvolvimento e validação de metodologia por Cromatografia Líquida de Alta Eficiência (CLAE) para pesquisa de enrofloxacina e ciprofloxacina em dejetos de poedeiras

• Ano de defesa: 2019
• Autor(a) principal: Thais Michelle Liziere da Silva
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: fluorquinolonas; dejetos de poedeiras; ambiente; Antibioticos em veterinaria; Antimicrobianos; Cromatografia líquida de alta eficiência Tese; Ave poedeira
• Link de acesso: http://hdl.handle.net/1843/SMOC-BBHFD3

The great advances in the poultry farming field have made Brazil a major egg producer. The search for the maximum productive efficiency in poultry farming leads to a continuous aggregation of new technologies by the sector. In this sense, the use of antimicrobials and chemotherapeutics contributes to the search for a more profitable activity. However, the use of such substances for the control of pathogens and parasites during the poultry breeding has been a cause of concern, mainly due to the increase of antimicrobial resistance. Considering that an important elimination route of these substances from the body is the poultry excreta, the environmental risks and impacts that may be caused are even greater. Despite this, there are no studies in Brazil dedicated to investigate the occurrence of antimicrobials in laying hens manure. The first step to the accomplishment of these studies is the development and validation of sensible and reliable analytical methodologies. Thus, the aim with this work is to develop and validate a method using HPLC-FD for the analysis of one of the most used fluoroquinolones in the laying hens farming, the enrofloxacin, and its active metabolite, the ciprofloxacin, in laying hens manure. The performance parameters analyzed for the validation were linearity, selectivity, matrix effect, precision, accuracy, limit of detection (LOD) and quantification (LOQ) and robustness for both analytes. The analytical methodology by HPLC-FD was validated and allowed to simultaneously analyze both antimicrobials studied, in a relatively short time, using a fast and simple extraction procedure. The method presented suitable linearity, selectivity and accuracy, with average recoveries of 92.95% and 94.07% for ciprofloxacin and enrofloxacin, respectively. The LODs found were 3.17 g kg-1 for ciprofloxacin and 3.33 g kg-1 for enrofloxacin, while the LOQs were 9.60 g kg-1 e 10.10 g kg-1, for ciprofloxacin and enrofloxacin, respectively. The method was robust for the analysis of ciprofloxacin, but it did not present robustness for the analysis of enrofloxacin, being advisable not to make changes in the method before new validation. Thus, the method by HPLC-FD presents the performance features needed, being suitable for the search of residues of enrofloxacin and its metabolite, ciprofloxacin, in laying hens manure.