Diversidade molecular de Fosfolipases D da peçonha da aranha Loxosceles laeta peruana revelada por sequenciamento de nova geração e análise transcriptômica

Detalhes bibliográficos
Ano de defesa: 2016
Autor(a) principal: Priscilla Alves de Aquino
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Minas Gerais
Brasil
Programa de Pós-Graduação em Bioinformatica
UFMG
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: http://hdl.handle.net/1843/57471
Resumo: This study aims to analyze, by bioinformatic tools, the transcripts of the brown spider venom gland Loxosceles laeta to expand the phospholipase D database. Sequences found through the new generation of sequencing RNA-seq and transcriptome assembly of the program Trinity, were subjected to gene annotation that so they could be identified through searches of similarity against different databases. Then they were classified into functional categories and had their biological function evaluated from a phylogeneticstandpoint. The identification correlated to the venom of compounds promotes better understanding of the pathophysiological processes of envenomation cases, as well as tools for improvements in methods of prophylaxis and treatment for loxoscelism. This dissertation aimed to describe the venom proteins responsible for its major toxic activities, which are the PhospholipaseD, Sphingomyelinase D or Dermonecrotic Proteins. In this study, 19 putative Phospholipase D sequences were described from the venom gland of the spider L. laeta. From the analysis and description of the Phospholipases D expression from the venom and their molecular interaction, it is possible to improve tools for the study of mechanisms of action of dermonecrotic activity of these enzymes. In addition, the in-depth study of the expression of these enzymes favor future studies on the phylogeny of their proteins.