Origem dos genes das vias de secreção do sistema digestivo humano

• Ano de defesa: 2017
• Autor(a) principal: Fenícia Brito Santos
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: Vias metabólicas; Heterotrofia; Digestão; Evolução; Genômica; Bioinformática
• Link de acesso: http://hdl.handle.net/1843/BUOS-B95MDB

The processes that ensure the maintenance of life require energy and for all heterotrophic organisms the source of energy are the nutrients available in the environment. Since the origin of the first forms of life the processes and systems involved in obtaining and metabolizing food have been determinant in the evolutionary success of the species. The emergence of multicellular organisms with compartmentalized systemsallowed metazoans to accumulate energy and reallocate it for the development of other functions. In humans the main stages of digestion depends on salivary, gastric, pancreatic and biliary secretions. Although studies of comparative anatomy in metazoa are well documented and the systemic knowledge of several processes is reported in many databases, studies addressing the comparative genomics and the evolution of the components of this system are scarce. Here our goal was to study the sequential origin of the genes involved in heterotrophy using the human digestive system as model. For this, software-recognizable diagrams for the digestive system secretion pathways were createdbased on models available in KEGG Pathway database. The origin of each component was estimated using tools for homologous clustering and for lowest common ancestor inference. This allowed us to infer the origin of the system based on the origin of its genes. Our results show that the most ancestral genes found in the pathways act on cell signalingprocesses and arose before the systems have been originated. The most recent components, such as receptors and some transporters, which are essential for secretion function, appeared from Metazoa. In addition, some components with auxiliary function, such as bicarbonate secretion in the pancreas and bile, have a more recent origin,indicating that this process appears as a refinement in these secretory pathways. Salivary secretion has the highest number of recent components and many of the proteins secreted are exclusive in mammals. In addition, we performed an analysis using the ELDOgraphprogram to identify which organisms or OTUs have more proteins close to human. The results show that the species that has more ELDO with human belong to the genus Pan (chimpanzees and bonobos). Analyzing at the order level the species with more ELDO with human belong to the orders Dermoptera and Rodentia. Our results show that the secretion pathways of the digestive system in mammals share many similarities, although some proteins are more distant. The data presented here allowed us to draw a scenario about the evolution of the digestive process, contributing to the evolutionary history of this system