Alterações em cinética temporal da expressão de proteínas plasmáticas in vivo e da viabilidade celular in vitro, induzidas pelas radiações ionizantes

• Ano de defesa: 2016
• Autor(a) principal: Celso Vieira de Lima
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: Reprogramação; Viabilidade celular; Modulação; Radiação; Radioterapia; Engenharia nuclear
• Link de acesso: http://hdl.handle.net/1843/BUBD-AFGN9L

Human existence on planet Earth has as one of its foundations adaptation of our body to radiation, such as solar and fund specific to each environment in which we live. So due to radiation exposure, our body has undergone physiological changes that allowed a genomic stability. However, interactions of the radiation with living beings still require many studies and understanding. In this context, this study aims to evaluate the changes in kinetic time, plasma protein expression isogenic Wistar rats in the control group and whole body irradiated using source of cobalt-60 in doses of 2 and 5 Gy, and the Cell viability at different dose rates, and Peripheral Blood Mononuclear cells (PBMC), sensitive radio, and 10 Gy, 2, compared with the neoplastic epithelial lineage breast cancer MDAMB-234, radioresistant. To study Wistar rats 12 to 18 weeks of age, which were irradiated whole body Co-60 source, with a dose of 5Gy. After irradiation of the animals was collected volume of 0.3 ml of blood, which was removed by centrifugation total serum that was applied to an SDS-PAGE 10% gel. The results showed, in irradiated animals full body, that there is a significant change in the biochemical composition of the plasma and thus the blood, with a marked reduction of the protein albumin in the early hours (24-48 hours) after treatment and a possible partial recovery over time. also revealed modulation and reprogramming the expression of a number of other proteins such as globulins. Regarding the irradiated cells was observed to decrease the viability of neoplastic and normal cells subjected to treatment with high-dose (10 Gy), and a high cell viability of MDAMB-234 in lower dose (2 Gy), this viability effectively observed increased and when the doses rates were below the therapeutical (subclinical). These data suggest the existence of dynamic systems adjustments to exposure by radiation in cell viability and protein expression level, which could set the evolutionary mechanisms of adaptation to radiation.