Uso da citometria de fluxo para detecção de Babesia canis

• Ano de defesa: 2001
• Autor(a) principal: Kelly Alves Bicalho
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: Diagnóstico; Citometria de fluxo; Babesia canis; RIFI; Cães; Cão Doenças; Imunofluorescência; Babesiose em cão Diagnóstico
• Link de acesso: http://hdl.handle.net/1843/BUOS-8C4DZV

The technique of flow cytometry was evaluated for detection of infected erythrocytes with Banesa kennels. In the standardization of the technique have been tested and propidium lodeto Hidroetidina as markers of infected erythrocytes. Hidroetidina showed better results and was used in other experiments. Control dogs and experimentally infected with B. canis were followed by flow cytometry and blood smears, capillary and venous blood, for periods from 21 to 195 days. In smears the first infected erythrocytes were detected on the second day after inoculation, with peak parasitemia capillary and venous occurring during the first week. After this period, the presence of the parasite has become an inconsistent finding, with occasional periods of absence, have not obeyed a pattern of behavior.