Nível de mRNA dos interferons tipo I e III e de genes estimulados por interferon em PBMCs de pacientes com esclerose sistêmica

• Ano de defesa: 2011
• Autor(a) principal: Danilo Bretas de Oliveira
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: Microbiologia
• Link de acesso: http://hdl.handle.net/1843/BUBD-AC7EQQ

Objective: Systemic sclerosis (SSc) is a complex autoimmune disease in which interferons (IFNs) may play an essential role. We hypothesized that type I and III IFNs may be found in increased levels on patients and be responsible for SSc autoimmune status. Methods: Type I and III IFNs and ISGs basal expression profiles were measured by qPCR using RNA from patients and controls PBMCs. Results: Type I IFNs are increased in SSc patients, while no induction of type III IFNs was detected. This induction cannot be related to IRF7, since no upregulation of this gene was seen on patients. Of the ISGs tested, 25OAS levels were increased in patients, while 6-16 and MxA levels were not. Conclusions: While there is no indication of type III IFN induction, increased levels of type I IFNs may lead to abnormal regulation of ISGs that can be responsible for immune system alterations described for SSc.