Liberação de componentes orgânicos e resposta periodontal em relação a um sistema restaurador resinoso Bulk-Fill: estudo in vivo

• Ano de defesa: 2019
• Autor(a) principal: Tassiana Cançado Melo Sá
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; Brasil; ODONTO - FACULDADE DE ODONTOLOGIA; Programa de Pós-Graduação em Odontologia; UFMG
• Palavras-chave em Português: Resinas compostas; Ensaio clínico; Teste de materiais; Interleucinas; Metacrilatos
• Link de acesso: http://hdl.handle.net/1843/31175

Most composite resins present methacrylates as the main monomers of their composition. In composite resins, the release of methacrylate monomers, associated with the polymerization products, has been considered as the source of a series of biological reactions such as toxicity or pulp reactions. The objectives of this study were to evaluate the clinical performance of NCCL restorations with composite resin, to determine also the presence of IL-1β and IL-6 cytokines in the gingival crevicular fluid (GCF) and the release of resinous components to saliva. The FL-Bond II restorative system (adhesive system) / Beautifil Bulk (restorative composite resin) was used. A longitudinal clinical study was performed in vivo, where patients with a non-carious cervical lesion with a restorative need were selected. Anterior and posterior teeth with NCCL and sensitivity were designated as experimental group and the corresponding tooth as control group. Prior to the treatment, were performed periodontal evaluation, collection of saliva and gingival crevicular fluid. The lesions were restored and, after 10 minutes, 7 days, 1 month and 6 months, there was a clinical evaluation of the restorations according to the FDI criteria and the periodontal response were made. Additionally, in all of these evaluation periods there was collection of saliva and crevicular fluid were colleted. Saliva samples were analyzed by LC-MS in order to identify the possible presence of monomers. Crevicular fluid samples were analyzed using the ELISA method for identification and quantification of interleukins. To perform intra- and inter group comparisons of clinical parameters, the McNemar test for categorical variables and the Wilcoxon test for numerical variables were used. To compare the classification of the FDI criterion between the times, the Stuart-Maxwell test was used. For the analysis of saliva, the data of quantity of monomers released over time were submitted to Student's t-test and the relation with total amount released were correlated with the number of restorations and with the total volume collected from each restoration by means of Pearson's Correlation. The clinical performance of NCCL restorations of a bulk-fill resin restorative system by FDI criteria was considered satisfactory over 6 months. Considering the periodontal response of the surrounding tissue to the NCCL restorations, it was observed that periodontal clinical parameters Bleeding on Probing, Gingival Index and Prohibing on Depth were more pronounced around the restored teeth. The bulk-fill resin restorative material did not cause statistically significant changes in the volume of GCF and in the IL-1β and IL-6 cytokines around restored teeth in the evaluated sample. No Bis-GMA was detected in the saliva samples collected before, 01 and 06 months after. For the Bis-GMA, there was no statistical difference between the analyzed periods. The mean TEGDMA release before the restoration was statistically lower than after 10 minutes. The results of the present study suggest that NCCL restoration may affect periodontal clinical parameters, but it was not able to affect the release of cytokines and the volume of GCF. The release of Bis-GMA was not considered significant over 6 months. However, the release of TEGDMA was significant only 10 minutes after the restoration was performed.