Efeitos do hialuronato de sódio e dos nanotubos de carbono funcionalizados com hialuronato de sódio no reparo ósseo de alvéolos dentários de ratos diabéticos

• Ano de defesa: 2011
• Autor(a) principal: Marcos Augusto de Sa
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: Reparo ósseo; Diabetes mellitus; Hialuronato de sódio; Nanotubos de carbono; Alvéolos dentários; Biologia celular; Diabetes
• Link de acesso: http://hdl.handle.net/1843/BUOS-96MHA2

Diabetes mellitus alters the bone metabolism leading to a reduction in the rate of its remodeling. Sodium hyaluronate (HY) facilitates cell migration, adhesion, proliferation and differentiation. Additionally, carbon nanotubes (CNT) is also thought to promote the tissue repair by serving as nucleation site for deposition of bone matrix and cell proliferation. The aim of this study was to evaluate the effects of HY and CNT functionalized with HY (CNT-HY) on the bone repair of tooth sockets of rats with type I diabetes after extraction of first molars. Diabetes was induced by a single injection of streptozotocin (50 mg/Kg) in the penile vein. The surgeries were performed, the sockets filled with carbopol (vehicle), HY or CNT-HY and the animals were divided into the following groups: (i) normal control, (ii) diabetic control, (iii) diabetic treated with HY (1%) and (iv) diabetic treated with CNT-HY (100 µg/mL). Control animals were treated with carbopol. After seven and fourteen days, the rats were euthanized and the analysis of the sockets was performed by quantifying the percentage of bone formation and the number of cell nuclei. Fourteen days after the surgeries, the percentage of trabecular bone was markedly lower in sockets of diabetic control animals when compared with sockets of normal control animals. The treatment of sockets of diabetic animals with HY or CNT-HY significantly increased the deposition of bone trabeculae when compared with sockets of diabetic control animals. Moreover, the sockets of diabetic control animals showed a significantly increase in the number of cell nuclei in relation to normal control rats. The treatment of sockets of diabetic animals with HY or CNT-HY significantly reduced the number of cell nuclei when compared with diabetic control sockets. Thus, these results showed that the treatment with HY or CNT-HY accelerates the bone repair in tooth sockets of diabetic rats, suggesting that these biomaterials hold a potential therapeutic application in diabetic patients.