Ômega conotoxina MVIIC no tratamento do trauma medular agudo em ratos Wistar

• Ano de defesa: 2018
• Autor(a) principal: Sandro Coelho de Azevedo
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: estresse oxidativo; conotoxina; qRT-PCR; apoptose; Mascis impactor; peroxidação lipidica; Trauma medular agudo; MVIIC; Ciência animal
• Link de acesso: http://hdl.handle.net/1843/SMOC-B7DH72

Calcium influx is an important mechanism of secondary injury to acute medullary trauma (TMA). Studies with calcium channel blockers have been developed in order to establish new therapies to reduce lesions secondary to spinal cord trauma. Toxins derived from marine snail venom are shown as promising therapy by selectively inhibiting the calcium channels and preventing the influx of this ion, which would reduce the progression of the lesion. Thus, -conotoxin MVIIC has been used by selectively inhibiting calcium channels, preventing the progression of the lesion and may have a beneficial effect for the treatment of acute medullary trauma . The objective of this study was to evaluate neuroprotection factors such as lipid peroxidation, oxidative stress and cellular apoptosis, systemic effects of -conotoxin MVIIC, as well as the locomotor function of rats submitted to TMA and treated with MVIIC conotoxin. METHODS: 36 male Wistar rats weighing 450g were randomly assigned to six groups of six animals: positive control (CTL +), negative control (CTL -), TOX 20 pMOL 30 ', TOX 20 pMOL 2h, TOX 40 pMOL 30 ', TOX 40 pMOL 2h. All the animals underwent laminectomy of the T12 vertebra and TMA, except for the CTL group - who did not suffer the trauma. The lesions were produced according to the international protocol MASCIS (Multicenter Animal Spinal Cord Injury Study), with the release in free drop of 10 g weight impact, with a predetermined height of 12.5 mm on the dura mater for 3 seconds . After 30 minutes and 2 hours of initial trauma, CTL + received intrathecal injection of 10L of sterile PBS and TOX groups, equivalent volume of MVIIC toxin solution at the recommended concentration for each group. It was observed that locomotor recovery and neuroprotection mediated by reduction of the gene expression of pro-apoptotic factors and increased gene expression of anti-apoptotic factor by means of the qRT-PCR technique in addition to reduction of oxidative stress in rats treated with MVIIC after acute medullary injury.