Frequência de Streptococcus uberis em amostras de leite de rebanhos bovinos de Minas Gerais e identificação de seus fatores de virulência

• Ano de defesa: 2010
• Autor(a) principal: Rayane Amaral da Silva Moraes
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: fatores de virulência; Streptococcus uberis; mastite bovina; Leite Análise; Virulência (Microbiologia); Bovino de leite Doenças; Mastite Controle
• Link de acesso: http://hdl.handle.net/1843/SMOC-9HHN9E

Bovine mastitis causes the major economic losses in Brazilian dairy herds, and Streptococcus uberis is an important cause of this disease. Currently, the role of virulence factors of this pathogen in the interaction with the host has been the subject of research, in order to develop new strategies for prevention and control of bovine mastitis. Given the increasing importance of S. uberis in milk production chain in Brazil, in this work the identification of S. uberis was made in milk samples from Minas Gerais herds, using the PCR technique with the extraction of genomic DNA directly from milk. In 23 (9.2%) of 250 milk samples analyzed the presence of S. uberis was detected. The presence of genes pauA and sua, which respectively encode the virulence factors PauA and SUAM, was verified in 28 samples of S. uberis from different Minas Gerais southern region herds. These samples were also tested for antimicrobial susceptibility. pauA gene was detected in 22 (78.57%) of 28 samples, and the gene sua was found in 19 (67.86%) samples. All samples were sensitive to Cephalothin and Florfenicol, but a high degree of resistance to Tetracycline was found.