Expressão de genes de arroz homólogos a genes de arabidopsis relacionados à produtividade de grão

• Ano de defesa: 2015
• Autor(a) principal: Oliveira, João Augusto Vieira de
• Orientador(a): Brondani, Claudio
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Programa de Pós-graduação em Biologia (ICB)
• Departamento: Instituto de Ciências Biológicas - ICB (RG)
• País: Brasil
• Palavras-chave em Português: Expressão gênica; Produtividade de grão; Genes homólogos
• Palavras-chave em Inglês: Gene expression; Grain yield; Homologous genes
• Área do conhecimento CNPq: CIENCIAS BIOLOGICAS::GENETICA
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/6637

Evidences indicate that by 2030 an increase in 40% of rice production is necessary so that it can meet the demand of the world population. The aim of this study was to quantify the expression of rice genes homologs to Arabidopsis genes previously related to yield (RUBISCO, AVP1, DA1 and TOR) by RT-qPCR analysis. The genotypes used in the study were the upland rice cultivars BRSMG Curinga and Primavera, and the old variety Douradão, which were evaluated in a yield experiment under two soil fertility levels, in a factorial scheme, in completely randomized design with two replications. Plant tissue samples were collected in vegetative and reproductive stages, which were used for total RNA isolation and subsequent cDNA syntheses. The cDNA was then subjected to RT-qPCR to evaluate the expression of four genes studied. Significant differences were observed in the expression of RUBISCO gene in leaves of upland rice plants in the vegetative stage, where there was a higher expression in high-level fertility in the soil, while in the reproductive stage, there was a higher expression in the low fertility treatment. DA1 that negatively regulates cell proliferation was less expressed in the vegetative stage in the treatment with high level of fertility, suggesting the suppression of this gene. AVP1 was more expressed in the reproductive stage, probably in order to increase the availability of P in a fundamental phase for the formation of the grain. TOR was the most expressed gene in this work, with a greater expression in adequate conditions of fertility, confirming its action under favorable conditions of cultivation. This study indicates that even two species that diverged over 120 million years have conserved productivity related metabolic routes. Thus, genes previously studied and validated in the model species Arabidopsis and that are of interest to economically important crops such as rice, may be the starting point for the development of cultivars with higher performance and better agronomic traits.