Caracterização molecular de Mycobacterium tuberculosis isolados de pacientes atendidos na cidade de Goiânia-GO, pela técnica de RFLP-IS6110

• Ano de defesa: 2008
• Autor(a) principal: SANTOS, Lorena Cristina
• Orientador(a): KIPNIS, André
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Mestrado em Medicina Tropical
• Departamento: Medicina
• País: BR
• Palavras-chave em Português: Tuberculose; RFLP-IS6110; 1. Tuberculose Pacientes - Goiânia(GO) 2. Tuberculose Tratamento. 3. RFLP-IS6110
• Palavras-chave em Inglês: Tuberculosis; RFLP-IS6110
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tde/1826

Tuberculosis is a serious public health problem all over the world. It has been demonstrated that different M. tuberculosis strains, characterized by the RFLP-IS6110 standard technique, have different virulence properties and antibiotic resistance. The aim of this study was to characterize M. tuberculosis strains isolated from patients attending two state reference hospitals of Goiânia-Goiás, using the RFLP-IS6110 technique. Positive cultures of M. tuberculosis sampled and isolated from January 2006 to June 2007 had their DNA extracted. A total of 142 viable DNA samples were analyzed, of which 126 samples presented an RFLP-IS6110 profile. Similarities comparisons between samples, as well as with literature reported profiles, were done with Bionumerics software (version 4.0). Forty three percent of the samples could be grouped in 24 clusters, when analyzed by the RFLP method, suggesting recent transmission among individuals belonging to the same cluster, however those patients did not present any epidemiological relationship, suggesting possible casual transmission between members of the same cluster. When compared with strain profile of the MDR-TB, three samples had grouped in the clade formed by families virulent. This study strengthens the importance of determining transmission routes not only within the state of Goiás but in the entire country, since there is the possibility of resistant strains transmissions.