Hipoexpressão de miR-205ab e miR-218a associada ao pior prognóstico em pacientes com câncer de mama

Detalhes bibliográficos
Ano de defesa: 2018
Autor(a) principal: Paes, Juliana Fracalossi lattes
Orientador(a): Saddi, Vera Aparecida lattes
Banca de defesa: Alves, Rosane Ribeiro Figueiredo, Santos, Silvia Helena Rabelo, Santos, Megmar Aparecida Carneiro
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Goiás
Programa de Pós-Graduação: Programa de Pós-graduação em Ciências da Saúde (FM)
Departamento: Faculdade de Medicina - FM (RG)
País: Brasil
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/9265
Resumo: Introduction: MicroRNAs are small molecules of single-stranded non-coding RNAs that regulate gene expression in the post-transcriptional phase and are being evaluated as prognostic and predictive markers in breast cancer. Objective: The aim of this study was to evaluate the expression of hsa-miR-205ab and hsa-miR-218a microRNAs and BRCA1 protein in breast cancer samples and their associations with the pathological and prognostic clinical aspects of tumors. Method: A group of 92 breast cancer cases was evaluated for clinical-pathological aspects, five-year survival, expression of BRCA1, hsa-miR-205ab and hsa-miR-218a. BRCA1 expression was assessed by immunohistochemistry and the quantification of the miRNAs by quantitative PCR in real time. The clinical-pathological aspects were compared in relation to the expression of BRCA1, hsa-miR- 205ab and hsa-miR-218a using the Mann-Whitney test. Survival curves were generated by the Kaplan-Meyer method and compared by the long-rank method. Results: The group of tumors comprised 56 cases of non-triple-negative breast carcinomas and 36 tumors with triple-negative phenotype. Hypoexpression of hsa-miR-205ab and hsa-miR-218a was associated with larger tumors (> 2 cm), presence of lymph node metastasis and distant metastasis, the highest histological grade, triple negative phenotype, absence of expression of BRCA1 and lower survival. (P = 0.044), more advanced stages (p = 0.005), lymph node involvement (p = 0.038), presence of distant metastasis (p = 0, 0008) and absence of BRCA1 expression (p = 0.039). Conclusion: The hypoexpression of hsa-miR-205ab and hsa-miR-218a was associated with factors of poor prognosis, absence of BRCA1 expression and the lowest survival rate in breast cancer patients evaluated in this study.