Detalhes bibliográficos
| Ano de defesa: |
2015 |
| Autor(a) principal: |
Ribeiro Júnior, Eli José Miranda
 |
| Orientador(a): |
Gil, Eric de Souza
|
| Banca de defesa: |
Gil, Eric de Souza,
Santiago, Mariângela Fontes,
Lobón, Germán Sanz |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Federal de Goiás
|
| Programa de Pós-Graduação: |
Programa de Pós-graduação em Engenharia do Meio Ambiente (EEC)
|
| Departamento: |
Escola de Engenharia Civil - EEC (RG)
|
| País: |
Brasil
|
| Palavras-chave em Português: |
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| Palavras-chave em Inglês: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
http://repositorio.bc.ufg.br/tede/handle/tede/4745
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Resumo: |
Laccase is a poliphenoloxidase enzyme that catalyzes the oxidation of phenolic compounds in the corresponding quinones. The current obtained in this redox process can be used for quantitative analysis. In this work, a carbon paste biosensor modified gluteraldehyde functionalized silica and an enzymatic extract of the “Pycnoporus sanguineus” fungi as a lacase source is proposed for phenol determination. The effect of carbon paste and electrolyte composition, pH from 3,0 to 8,0, start potential from 0,55 to 0,25mV, scan rate from 5 to 25 mV s-1 and potential pulse amplitude from 10 to 60mV on the differential pulse voltammetric response was investigated. A linear correlation of R² = 0,9946 was obtained for the phenol content (catechol) in the concentration range from 50 to 500nMol L-1, with a detection limit of 30nMol L-1. This biosensor was used for the determination of different kinds of phenolic compounds, presenting a better response for catechol. |
