Utilização da RMN e ferramentas quimiométricas na avaliação das alterações metabólicas de Citrus sinensis (L.) Osbeck (var. caipira) causadas por Xanthomonas axonopodis pv. citri

• Ano de defesa: 2010
• Autor(a) principal: SILVA, Lorena Mara Alexandre e
• Orientador(a): ALCANTARA, Glaucia Braz
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Mestrado em Química
• Departamento: Educação em Química
• País: BR
• Palavras-chave em Português: RMN; Quimiometria; Xanthomonas axonopodis; Citrus sinensis; 1.RMN 2. Quimiometria 3. Xanthomonas axonopodis pv. Citri
• Palavras-chave em Inglês: NMR; Chemometrics; Xanthomonas axonopodis; Citrus sinensis
• Área do conhecimento CNPq: CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tde/1037

The NMR and chemometrics was used and show that is important to exploratory analysis of monitoring and understanding of the pathogenic action caused to Xanthomonas axonopodis pv. citri bacteria. In the extracts, the morphological characteristics were more important in PCA composition and in HR-MAS analysis, the metabolic variations were more important in PCA characterization, especially carbohydrates. Thus, there was the variation especially of carbohydrates, amino acids and organic acids in regions of lower chemical shift, which AABA can be considered an inherent compound of plant defensive response. In orange juices, inherent compounds of fermentation processes have increased their production demonstrating that the bacterial infection has caused an increase in the rate of fruit decay. Flavonoids also have showed the variation of their metabolism and in many cases, there were reduction of their production, which were led to loss of important compounds for farmacoligic characteristics of orange leaves. However, important flavonoids like hesperidin and naringenin remained constant during bacterial infection. HR-MAS data corroborated those results obtained in solution NMR analysis, indicating that both techniques are complementary to more comprehensive analysis, since differences in volatile terpenoids production, for example, were easily visualized in matrices without pretreatment through HR-MAS NMR. The biological evaluations have shown that NMR can be a tool in the design of experiments with the extracts monitoring. The trend of antioxidant activity data was similar with PCA of extracts, which the correlation between the results obtained from both techniques confirmed the contribution of amino acids as important compounds to defensive system of Citrus against the pathogenic attack.