Efeitos de argentilactona sobre o perfil transcricional, parede celular e estresse oxidativo de Paracoccidioides spp

• Ano de defesa: 2014
• Autor(a) principal: Araújo, Felipe Souto
• Orientador(a): Pereira, Maristela
• Banca de defesa: Pereira, Maristela, Schrank, Augusto, Cravo, Pedro Vitor Lemos, Soares, Célia Maria de Almeida, Rocha, Juliana Parente
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Programa de Pós-graduação em Genetica e Biologia Molecular
• Departamento: Instituto de Ciências Biológicas - ICB (RG)
• País: Brasil
• Palavras-chave em Português: Paracoccidioides spp; Argentilactona; Transcritoma; Antifúngico
• Palavras-chave em Inglês: Paracoccidioides spp; Argentilactone; Transcriptome; Antifungal
• Área do conhecimento CNPq: GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/4173

Paracoccidioides spp, dimorphic pathogenic fungi, is the etiologic agent of paracoccidioidomycosis (PCM). PCM is an endemic disease that affects at least 10 million people in Latin America, causing severe public health problem. The drugs used against pathogenic fungi have various side effects and have limited efficacy, therefore there is an inevitable and urgent medical needing for the development of new antifungal drugs. In the present study, we evaluated the transcriptional profile of Paracoccidioides spp exposed to argentilactone, a constituent of the essential oil of Hyptis ovalifolia. A total of 1,058 genes were identified, of which 208 were up-regulated and 850 down-regulated. The cell rescue, defense and virulence, with a total of 26 genes, was a functional category with a large number of genes induced, among them, heat shock protein 90 (hsp 90), cytochrome c peroxidase (ccp), hemoglobin ligant RBT5 (rbt5) and superoxide dismutase (sod). Quantitative real-time PCR revealed an increase in the expression level of all those genes. Enzymatic assay showed a significant increase in SOD activity. The reduced growth of Pbhsp90-aRNA, Pbccp-aRNA, Pbsod-aRNA, Pbrbt5-aRNA isolates in the presence of argentilactone indicates the importance of these genes in Paracoccidioides spp responding to argentilacone. Paracoccidioides spp cell wall was also evaluated. The results showed that argentilactone causes a decrease in the levels of polymers of the cell wall. These results suggest that argentilactone is a potential candidate for antifungal therapy.