Detalhes bibliográficos
| Ano de defesa: |
2021 |
| Autor(a) principal: |
Wakui, Vinícius Galvão
 |
| Orientador(a): |
Kato, Lucilia
|
| Banca de defesa: |
Kato, Lucilia,
Queiroz Júnior, Luiz Henrique Keng,
Oliveira, Cecilia Maria Alves de,
Alcantara, Glaucia Braz,
Lago, João Henrique Ghilardi |
| Tipo de documento: |
Tese
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Federal de Goiás
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| Programa de Pós-Graduação: |
Programa de Pós-graduação em Química (IQ)
|
| Departamento: |
Instituto de Química - IQ (RG)
|
| País: |
Brasil
|
| Palavras-chave em Português: |
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| Palavras-chave em Inglês: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
http://repositorio.bc.ufg.br/tede/handle/tede/11877
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Resumo: |
The Brazilian Cerrado is recognized for its biodiversity and the presence of several endemic species with a great potential for biological activities. The specie Lomatozona artemisiifolia Baker is endemic from Cerrado of the Brazilian Midwest region and it is currently endangered. The in vitro micropropagation studies were conducted in previous works, a technique that allows its production in the laboratory looking for conservation on this biome. Thus, based on the biological activities already reported in the literature for other species of Asteraceae endemic to the Cerrado, this work reports the phytochemical study of L. artemisiifolia and a comparison between in vitro and the native species of this biome. The observation of missing chemical study with species of the genus Lomatozona, the major compounds labdan-8-ol-15-il (1), labdan-8,15-diol (2), 8-hydroxylabdan-15-oic acid (3), chrysosplenetin (4), paquipodol (5), quercetagetin-3,5,6,3',4'-pentamethyl ether (6) and oxyanin B (7), were isolated. Then, the extracts of L. artemisiifolia from native and in vitro species were studied by LC-ESI-MS/MS and they have been compared by the chemometric analysis, thats allow to observe significant differences between the ethyl acetate extracts, that was related to its foliar surface content. The molecular network analyzes were conducted in order to annotate the other compounds present in this species, leading to dereplication of several subclasses of flavonoids. The chemometric analysis together with the molecular network analysis allowed us to identify the metabolite subclasses whose production is influenced by the conditions of field growth and in vitro micropropagation. It was observed that native and in vitro species are mainly differentiated by the chemical content secreted by glandular trichomes, distributed over the foliar surface of this species, thus indicating possible abnormalities in the development of vegetal structures in the plant obtained by micropropagation. The extracts from Lomatozoma were evaluated on the control of the phytonematode Pratylenchus brachyurus based on the metabolite classes identified in L. artemisiifolia. However, the results indicated a low potential for the nematicide activity. |
