Toxoplasmose gondii: soroprevalência, isolamento e virulência de cepas obtidas de galinhas caipiras (Gallus domesticus) comercializadas em feiras livres do município de Goiânia

• Ano de defesa: 2007
• Autor(a) principal: Aleixo, Eduardo da Costa Alves
• Orientador(a): Soares, Joanna Darc Aparecida Herzog
• Banca de defesa: Soares, Joanna Darc Aparecida Herzog, Gomes, Abraão Garcia, Bezerra, José Clecildo Barreto
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP)
• Departamento: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
• País: Brasil
• Palavras-chave em Português: Toxoplasma gondii; Galinhas caipiras; Epidemiologia
• Palavras-chave em Inglês: Free-range chicken; Epidemiology
• Área do conhecimento CNPq: CIENCIAS BIOLOGICAS::PARASITOLOGIA::PROTOZOOLOGIA DE PARASITOS
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/11404

In this study, 50 free-range chickens were obtained from free fairs of the city of Goiânia, its serum were tested for antibodies anti T. gondii with the modified agglutination test (MAT), its heart and brain had been processed and inoculated in groups of 6 mice. Samples of the organs of the birds and the surviving mice had been sectioned and stained with Hematoxylin-Eosin for the search of Toxoplasma-like cysts structures. The serum of the surviving mice was analyzed by the indirect immunofluorescence reaction (RIFI). The isolated ones had been submitted to the study of virulence for mice by inoculations with different concentrations of tachyzoites. 25 birds (50%) had revealed positive (MAT ≥ 1:5) getting a total of three isolated. Toxoplasma-like cysts structures had been found in histologic cuts of eight birds (16%) and organs of mice of two groups (2%). Of the 25 experiments with inoculeted of positives birds, in 11 (44%) it had the detention of IgG anti-Toxoplasma. In the samples of negatives birds, evidences of the presence of Toxoplasma had not been found. Of the three isolated, two had been lethal for mice from concentrations of a thousand tachyzoites., while another one was 100% fatal one from the concentration of ten a thousand tachyzoites.