Detalhes bibliográficos
| Ano de defesa: |
2018 |
| Autor(a) principal: |
Oliveira, Matheus Gabriel de
 |
| Orientador(a): |
Paula, José Realino de
|
| Banca de defesa: |
Paula, José Realino de,
Bara, Maria Teresa Freitas,
Alves, Suzana Ferreira,
Oliveira, Thiago Levi Silva |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Federal de Goiás
|
| Programa de Pós-Graduação: |
Programa de Pós-graduação em Ciências Farmacêuticas (FF)
|
| Departamento: |
Faculdade Farmácia - FF (RG)
|
| País: |
Brasil
|
| Palavras-chave em Português: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
http://repositorio.bc.ufg.br/tede/handle/tede/9745
|
Resumo: |
Hydrocotyle umbellata L., also known as "acariçoba", is an herb of the Araliaceae family native to the American continent. Recently, the antinociceptive, anti-inflammatory and anxiolytic-like effects of the specie were attributed to the lignan hibalactone found in the subterraneous parts of the plant. However, there is a lack of studies regarding its extraction process and analytical methods for its determination.This work aimed to develop and validate an HPLC-PDA analytical method for the determination of hibalactone in H. umbellata subterraneous parts and to apply experimental design techniques to optimize its ultrasound-assisted extraction (UAE). HPLC analysis were performed on a Waters® e2695 with PDA detector in a reversed-phase chromatography using acetonitrile/methanol/water (10:65:25), flow of 0.8 mL/min, detection at 290 nm and C18 column (250 mm x 4.6 mm, 5 μm). The primary validation parameters were determined according to Brazilian legislation. The optimization of the hibalactone UAE was performed using Box-Behnken desing and Response surface methodology (RSM) to investigate the influence of the following parameters: solid-to-liquid ratio, ethanolic strength and extraction temperature.The HPLC method proved to be selective, linear, precise, accurate and robust, being useful for the analysis of hibalactone in H. umbellata subterraneous parts extracts. The optimal UAE condition was obtained with solid-to-liquid ratio of 1:5 g/mL, ethanolic strength of 70% (v/v) and temperature of 65 °C. The experimental values of hibalactone content under optimal UAE conditions were in good agreement with the RSM predicted values. In this study, it was possible to develop and validate HPLC analytical method for the determination of hibalactone in H. umbellata subterraneous parts and to determine its optimal UAE conditions for application in quality control and standardization of raw materials of the specie. |
