Paper spray ionization: análise direta de licores do processo de etanol 2G por espectrometria de massas

Detalhes bibliográficos
Ano de defesa: 2015
Autor(a) principal: Carvalho, Thays Colletes de lattes
Orientador(a): Vaz, Boniek Gontijo lattes
Banca de defesa: Vaz, Boniek Gontijo, Abdelnur, Patrícia Verardi, Romão, Wanderson, Coltro, Wendell Karlos Tomazelli
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Goiás
Programa de Pós-Graduação: Programa de Pós-graduação em Química (IQ)
Departamento: Instituto de Química - IQ (RG)
País: Brasil
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/4915
Resumo: 2G Ethanol production is a laborious process that may result in formation of glucose -fermenting yeasts, which reduces the process yield. The search for new rapid and sensitive methods are required to show the condition with the formation of free sugars and absence of fermentation inhibitors (Hidroximetilfurfural and furfural). The present work proposes utilizing the Paper Spray (PS) Ionization source as a method of detecting and quantifying fermentation yeasts inhibitors and sugars generated in 2G ethanol-acquiring processes. The method was optimized and was obtained as calibration curves for the glucose obtained by enzymatic hydrolysis process. These conditions were used for analysis actual sample of sugarcane bagasse liquors. The best analysis conditions by PS were: distance between 1 and 5 mm, dry spot application mode, Whatman Grade 1 paper, 60º angle, modified paper with paraffin barrier, 10 μL volume and sample application concentration of 0,1mg/mL. The detection of the expected product in the pretreatment and hydrolysis liquors of sugarcane bagasse was performed as expected. The paper with paraffin channel proved to be more effective for the quantification of glucose with improved linearity (0.9952) and limits of detection (2.77 mmol/L) and quantification (9.27 mmol/L) compared to without changing paper and paper cur ends. Applying the PS prototype in determining sugars and inhibitors in sugarcane bagasse liquors has proven to be efficient and quick, opening up a perspective of utilizing this method on monitoring Ethanol 2G production processes.