Hanseníase: comparação entre testes rápidos com antígeno PGL-I em amostras de sangue-total e soro

• Ano de defesa: 2008
• Autor(a) principal: Grassi, Adriano Badotti
• Orientador(a): Stefani, Mariane Martins de Araújo
• Banca de defesa: Stefani, Mariane Martins de Araújo, Buhrer, Samira, Oliveira, Maria Leide de W.
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP)
• Departamento: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
• País: Brasil
• Palavras-chave em Português: Hanseníase; Sorologia anti-PGL-I; Teste rápido
• Palavras-chave em Inglês: Leprosy; α-PGL-I serology; Rapid test
• Área do conhecimento CNPq: CIENCIAS BIOLOGICAS::IMUNOLOGIA
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/6354

Objectives: This study compared two leprosy rapid tests detecting (1) IgM against PGL-I (ML-Flow test, KIT, Amsterdam, Netherlands) or (2) IgM, IgG and IgA against PGL-I (ML-ICA, Yonsei University, South Korea). Methods: Whole blood and serum from untreated multibacillary (MB; n=50) and paucibacillary (PB; n=48) leprosy patients (Reference Center for Diagnosis and Treatment, Goiania, central Brazil), MB household contacts (HHC; n=35) and healthy endemic controls (EC; n=50) were tested. Leprosy patients were classified by dermato-neurological evaluation, bacillary index (BI) and histopathology. Median age was 29 years, 39.9% males. MB patients had BB-BL-LL leprosy (median BI=2.0), PB patients had TT-BT leprosy. Results: (1) Using sera, ML Flow positivity of MB patients was comparable to ML-ICA positivity. (2) For ML-Flow tests, the positivity was comparable with whole blood and serum. (3) Among PB leprosy ML- flow positiviy was comparable for whole blood and serum but it was higher than the ML-ICA seropositivity in whole blood and serum. (4) Among MB leprosy patients positivity of ML Flow test in serum and whole blood was comparable to the positivity of ML-ICA in serum. Conclusion: ML-Flow test is more field applicable than ML-ICA since high positivity was observed among MB patients using whole-bood.