Avaliação do potencial do peptídeo indolicidina na descontaminação de sêmen bovino

• Ano de defesa: 2021
• Autor(a) principal: Santos, Gabriel Henrique
• Orientador(a): Souza, Guilherme Rocha Lino de
• Banca de defesa: Souza, Guilherme Rocha Lino de, Gambarini, Maria Lucia, Ferreira Júnior, Álvaro
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Programa de Pós-graduação em Ciência Animal (EVZ)
• Departamento: Escola de Veterinária e Zootecnia - EVZ (RMG)
• País: Brasil
• Palavras-chave em Português: BoHV-1; Leptospira; Peptídeos bioativos; Inativação viral
• Palavras-chave em Inglês: Bioactive peptides; Viral inactivation
• Área do conhecimento CNPq: CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/13625

The growing interest in artificial insemination in cattle has led researchers to study techniques that make it possible to preserve semen without compromising its ability to fertilize. This project evaluated the antiviral and antibacterial activity of the peptide indolicidin and its potential use as an antiviral agent for a product of high commercial value, which is the semen of selected cattle. Firstly, the antibacterial activity of the indolicidin peptide was evaluated against the bacterium E. coli at the concentrations described in the literature. Based on Escherichia coli, the antibacterial activity of the peptide was evaluated at concentrations of 10 μM, 20 μM and 30 μM against Leptospira interrogans. Subsequently, the peptide was tested at concentrations of 10 μM, 20 μM and 30 μM on cultured cells (MDBK), in which no changes in cell morphology or viability were observed. The antiviral activity of the peptide was then analyzed in MDBK cells artificially infected with bovine alphaherpesvirus type-1 (BoHV-1), in which the peptide was incubated at concentrations of 4 μM, 6 μM, 8 μM, 10 μM, 20 μM and 30 μM, and the safety of the peptide against bovine semen was evaluated according to quality parameters. The indolicidin peptide was safe against semen and cultured cells. In bacteria, the best concentration identified was 30 μM, while in BoHV-1 the best concentration was 8 μM. To date, no methodology has been described for the efficient inactivation of BoHV-1, nor even for minimizing the viral concentration in bovine semen, which makes this project interesting for the commercialization of semen on the national and international markets.