Estudo comparativo entre dois critérios de classificação histológica, contagem de agnor’s e expressão de c-kit no mastocitoma canino

• Ano de defesa: 2013
• Autor(a) principal: Borges, Juliana Carvalho de Almeida
• Orientador(a): Araújo, Eugênio Gonçalves de
• Banca de defesa: Araújo, Eugênio Gonçalves de, Miguel, Marina Pacheco, Damasceno, Adilson Donizeti
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Programa de Pós-graduação em Ciência Animal (EVZ)
• Departamento: Escola de Veterinária e Zootecnia - EVZ (RG)
• País: Brasil
• Palavras-chave em Português: Cães; Graduação histológica; Kiupel
• Palavras-chave em Inglês: Dogs; Histologic grading
• Área do conhecimento CNPq: CLINICA E CIRURGIA ANIMAL::CLINICA CIRURGICA ANIMAL
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/3375

In dogs, mastocytoma is the most common skin cancer, representing up to 21% of all skin tumors. Although the most consistent prognostic factor in diagnosis is histopathological evaluation, different classification systems for canine cutaneous mast cell tumors are used, reducing the prognostic value of histological grading. The Patnaik system, based on a three-level classification, is still the most used, despite its subjectivity. In 2011, KIUPEL and collaborators proposed a new histological classification system, sorting the tumors into two classes, low intensity and high intensity. The objective of this study was to evaluate the comparison between the methods of classification of Patnaik and Kiupel, and establish a possible relationship between the biological behavior of Kiupel method classified mastocytoma with the through the histochemical AgNOR's technique and the expression of c-KIT. Samples of canine cutaneous mastocytoma from the Laboratory of Animal Pathology, School of Veterinary and Animal Science, Federal University of Goiás were used for this purpose. In addition to highlighting the subjectivity of tumors classified as intermediate grade system by Patnaik, the analysis indicated that the Kiupel’s method brought up greater agreement among pathologists, confirming its efficiency. The proliferative index determined by AgNOR's was greater in high intensity mastocytomas. In general, the expression of c-KIT corroborated the histological classification. The results showed that the new Kiupel classification system, is more efficient for the histological diagnosis and prognosis of canine mastocytoma when coupled with immunohistochemical and histochemical study.