Interleucina-17 na atividade de fagócitos inflamatórios de camundongos BALB/c

• Ano de defesa: 2019
• Autor(a) principal: Lucio, Fernanda Hélia
• Orientador(a): Oliveira, Milton Adriano Pelli de
• Banca de defesa: Oliveira, Milton Adriano Pelli de, Gomes, Clayson Moura, Celes, Mara Rúbia Nunes
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Programa de Pós-graduação em Biologia da Relação Parasito-Hospedeiro (IPTSP)
• Departamento: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
• País: Brasil
• Palavras-chave em Português: IL-17A; IL-17F; IL-4; Macrófagos inflamatórios; IL-4 KO
• Palavras-chave em Inglês: Inflammatory macrophages
• Área do conhecimento CNPq: CIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::MICOLOGIA
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/11823

Introduction: Inflammatory macrophages express high amount of IL-17 receptor (IL17R) favoring IL-17 binding. The role of IL-17 activating macrophages to increase microbicidal activity or regulatory response is not completely clear, but previous studies demonstrated that IL-17 activates peritoneal macrophages to increase arginase and induced nitric oxide synthase. Here, we investigated the role of IL-17 and its interaction with IFN-γ, IL-4 and LPS to induce M1 or M2 profiles in wild type (WT) and IL-4 knockout (KO) BALB/c mice. Objective: Evaluate the ability of IL-17, alone or in association with other cytokines, to activate different macrophage profiles. Methods: Thioglycolate-elicited peritoneal macrophages were characterized by cytometry and stimulated in vitro for 48h with different association of IL-17A, IL-17F, LPS, IL-4 and IFN-γ. The supernatant was used to evaluate NO production and lysed cells were used to evaluate arginase activity. Results: Cells harvested from different days after thioglycolate inoculation showed no variation for M1/70, F4/80 and IL-17R. Adherent cells obtained on day 5 presented the largest number of M1/70, F4/80, IL-17R positive cells. IL-17 alone did not induce arginase activity or NO production in WT and IL-4 KO mouse macrophages. Association of IL-4 with IL-17 did not induce arginase activity, but IL-17 plus IFN-γ increased NO production in both strains tested. IL-4 KO macrophages presenting high arginase activity even without stimulation with cytokines and IL-4 increased in these macrophages.