Polimorfismo rs7903146 do gene TCF7L2 e sua associação com alterações da homeostase da glicose em crianças da Grande Vitória

• Ano de defesa: 2018
• Autor(a) principal: Pinto, Iara Almeida
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Mestrado em Biotecnologia; Centro de Ciências da Saúde; UFES; Programa de Pós-Graduação em Biotecnologia
• Palavras-chave em Português: Children; Glucose homeostasis; Obesity; TCFL2; SNP; Crianças; Homeostase glicêmica; Obesidade; Polimorfismo (Genética); Equilíbrio hidro-eletrolítico (Fisiologia); Obesidade em adolescentes; Biotecnologia; 61
• Link de acesso: http://repositorio.ufes.br/handle/10/10522

Overweight young people are more likely to become obese and diabetic. There is recent evidence suggesting that genes that alter glucose homeostasis are good candidates for influencing body weight the opposite is true. TCF7L2 is the main gene associated with DM2. Polymorphisms in this gene are directly related to the development of DM2 and possibly to body weight. For this reason, genotype frequencies were obtained for the rs7903146 polymorphism in the TCF7L2 gene in adolescents and it was verified if any genotype and / or genetic model was associated with parameters of glucose homeostasis and overweight. Adolescents enrolled in state public schools in the metropolitan region of Vitória-ES, from a representative sample were randomly included in the genetic study. The anthropometric evaluations and blood collection, after a 12-hour fast, were performed at the school itself. For classification of nutritional status, the body mass index / age (imc / i) index was considered in z score. The genomic DNA was extracted from peripheral blood samples. For the TCF7L2 gene, the DNA was amplified by allele-specific polymerase chain reaction and the products of this reaction were analyzed by polyacrylamide gel electrophoresis (12%), visualized by staining in 0.1% silver nitrate. Statistical analysis was performed in software spss version 23.0. Data from individuals regarding race, sex, age, imc / i, glycemia and HOMA-ir, HOMA-b% and HOMA -s% were analyzed. 312 adolescents (age: 10-14 years) divided into non-overweight (n = 223) and overweight (n = 89) groups were investigated. The frequencies for the dominant (TT + CT x CC) and recessive (CC + CT x TT) genetic models were also analyzed, but only a marginal association (p = 0.090) with imc / i was found for the dominant model. HOMA - β% l v ls w r sso t w th notyp s n the overweight group, with lower values found in children with a CT or TT genotype (p = 0.034) also observed in the dominant model (CT + TT p = 0.013). The results suggest that the association with HOMA-β m y b pr tor of early failure in insulin secretion and possibly dependent on BMI.