Análise proteômica de tecido cardíaco de ratos com diferente capacidade aeróbica intrínseca

• Ano de defesa: 2012
• Autor(a) principal: Ribeiro, Leonardo Perin
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Mestrado em Ciências Fisiológicas; Centro de Ciências da Saúde; UFES; Programa de Pós-Graduação em Ciências Fisiológicas
• Palavras-chave em Português: Química de proteínas; Fisiologia; 61
• Link de acesso: http://repositorio.ufes.br/handle/10/7978

Exercise capacity is a complex attribute that involves different physiological systems under the influence of both genetic and environmental factors. Related to genetic influence, results have shown that more than 70% of aerobic exercise capacity is intrinsically determined. In this work, a comparative proteomic approach, twodimensional gel electrophoresis (2D) combined with MALDI-TOF/TOF tandem mass spectrometry, was used to investigate possible molecular differences at the protein expression level between rats heart (left ventricle - LV) with distinct intrinsic exercise capacity. Low running performance (LRP) and high running performance (HRP) rats were categorized by a maximal exercise test protocol performed on a motor-driven treadmill, according to total distance performed (TDP). The running capacity of HRPs was 3.5 fold greater than LRPs. Protein expression profiling revealed 29 statistically significant (p<0,05) differences between HRP and LRP, and 15 of these proteins were identified by MALDI-TOF/TOF (MS and MS/MS). Robust alterations were detected in components involved in antioxidant and stress response, miofibrillar and cytoskeletal proteins. Contractile proteins were found to have special expression modification: α-myosin heavy chain-6, myosin light chain-1 and creatine kinase up regulation in LV of HRP rats on patterns in HCR. In contrast, LV of LRP rats exhibited increase in abundance of protein associated with stress response. These animals exhibited enhanced expression of the antioxidant enzyme (aldehyde dehydrogenase 2), and heat shock proteins (α-crystallin B chain, heat shock protein β-2). In addition, the cytoskeletal proteins, desmin and α-actin, were upregulated in LCRs. In conclusion, our results suggest that the increased contractile proteins levels in HCRs rats may explain, in part, the improved exercise capacity. The increased stress protein expression in LCR suggests that the LV proteome of these animals are exposed to greater stress