Estudo químico e biológico de Hippeastrum puniceum (Lam.) Kuntze (Amaryllidaceae)

• Ano de defesa: 2017
• Autor(a) principal: Soprani, Letícia Carlesso
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Mestrado em Ciências Farmacêuticas; Centro de Ciências da Saúde; UFES; Programa de Pós-Graduação em Ciências Farmacêuticas
• Palavras-chave em Português: Alkaloids; Antioxidant; Thrombolytic; Hippeastrum puniceum; Amaryllidaceae; Trombolítico; Amarilidácea; Antioxidantes; Alcalóides; Farmácia; 615
• Link de acesso: http://repositorio.ufes.br/handle/10/8360

Hippeastrum puniceum is a specie that belongs to Amaryllidaceae This family presents a very specific group of alkaloids known as Amaryllidaceae alkaloids, (group of isoquinoline alkaloids), showing several biological activities such as antioxidant activity, inhibition of the enzyme acetylcholinesterase, antiviral action, antifungal, antiparasitic, and others. The purpose of this work was to study the chemical composition of the bulbs of the species Hippeastrum puniceum (Lam.) Kuntz., and evaluate the possible biological activity. The acidbase extraction was performed for the methanolic extract of H. puniceum bulbs, obtaining 03 other fractions: Hp01 (Hexan), Hp02 (Ethyl Acetate) e Hp03 (Ethyl Acetate:Methanol 3:1). Then were used classics techniques of fractionation and purification. Tests were performed to evaluate the antioxidant activity against the synthetic radical DPPH and ABTS, as well as the determination of in vitro thrombolytic activity that evaluated the capacity of the extracts promote clot lysis. Analysis of the extracts by GC-MS revealed the presence of 11 alkaloids: 9-O-demethyllicoramine, licoramine, galantamine, assoanine, kirkine, 8- demethylmaritidine, pancratinine, 11-hydroxyvittatine, pseudolicorine, 2αhydroxyhomolycorine and licorine. They were isolated by standard techniques of isolation and purification of the alkaloids 9-O-demethyllycoramine, 9- demethyl-2α-hydroxyhomolycorine, licorine and tazettine. The fractions HpEb, Hp02 and Hp03 showed antioxidant capacity. The results obtained demonstrate the progress in the phytochemical study of Hippeastrum puniceum. However it is necessary to search for other methodologies to evaluate the biological potential of this species.