Caracterização química e avaliação da atividade fungicida dos óleos essenciais de quatorze espécies vegetais frente ao Fusarium solani f.sp. piperis e Thielaviopsis paradoxa

• Ano de defesa: 2019
• Autor(a) principal: Santos, Kézia Rayane Roncati dos
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Mestrado em Química; Centro de Ciências Exatas; UFES; Programa de Pós-Graduação em Química
• Palavras-chave em Português: Essential oils; Fungicidal activity; Cytotoxicity; Thielaviopsis paradoxa; Fusarium solani f.sp. piperis; Óleos essenciais; Atividade fungicida; Citotoxicidade; Fusarium solani; Thielaviopsis paradoxa.; Química; 54
• Link de acesso: http://repositorio.ufes.br/handle/10/11132

The loss of agricultural products due to phytopathogenic fungi is a matter of great impact in the world. Synthetic fungicides have been widely used in the control of fungal diseases. The application of essential oils is an alternative method of controlling fungal infections, justified by the toxicity and resistance of pathogenic fungi to syntheti c fungicides. The aimof this study was to find new antifungal agents among fourteenspecies of plants of the Brazilian Atlantic Forest. The species were hydrodistilled, and their respective yields were calculated, and the analysis for chemical characterizati on of their components was examined by GC-MS. Thecompositions of the essential oils were characterized by the predominant presence of larger amounts of sesquiterpenes, followed by hydrocarbon monoterpenes, indicated as responsible for the more effective inhibitory effect of volatile oils. Next, the antifungal properties determined by the microdilution method against Fusarium solanif.sp. piperisand Thielaviopsis paradoxa. All essential oils showed some inhibition against fungi tested. In vitro assays showed that the species of Siparuna brasiliensis, Campomanesia macrobracteolata(leaves and fruits), Piper miquelianumC. DC., Peperomia alata, Baccharis platypodaDC., And Lantana lundianaSchauer were the best for growth control of Fusarium solanif.sp. piperis, exhibiting inhibition concentrations of 800 µg mL-1. For the tests against Thielaviopsis paradoxa, the volatiles of Protiumsp. and Etlingera elatiorshowed low inhibition concentrations, considered as fungicides at the concentration of 200 µg mL -1. In relation to the cytotoxicity assays performed with murine macrophages (Raw 264.7) by the MTT reduction method, the essential oils did not present cytotoxicity at the concentrations which were considered fungicidal and inhibitory against T. paradoxaand F. solanif. sp. piperis, respectively. These results suggest that the oils studied in this work have the potential to be used in the control of fungal infections caused by these phytopathogens