Caracterização fenotípica de Fusarium guttiforme e Fusarium ananatum patógenos do abacaxizeiro

• Ano de defesa: 2017
• Autor(a) principal: Moura, Raissa Debacker
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Mestrado em Biotecnologia; Centro de Ciências da Saúde; UFES; Programa de Pós-Graduação em Biotecnologia
• Palavras-chave em Português: Taxonomy; Fungi; Microscopy; Citometry; Biotechnology; Taxonomia; Fungos; Ananas comosus; Microscopia; Citometria; Biotecnologia; 61
• Link de acesso: http://repositorio.ufes.br/handle/10/7088

Fusarium ananatum and Fusarium guttiforme are respectively the etiological agents of the black spot and fusariosis, important diseases of the pineapple culture. The two species of fungi are morphologically very similar but genetically distinct. This work aimed to identify and characterize phenotypic markers that can be used by the phytosanitary services of Brazil and other pineapple producing countries to identify the etiological agents of fusariosis and black spot. Were developed studies to characterize in vitro both species at different temperatures and culture media, besides characterizing and identifying new phenotypic markers. The ideal temperature for the development of the species was 25°C, and also the highest production of macroconidia was in the culture medium Basal Medium with Carboxymethylcellulose (BM+CMC) that obtained the production of 3,2x105 macroconidia/ml and 1,8x105 macroconidia/ml for F. ananatum and F. guttiforme, respectively. For the development of microconidia, the Synthetic Nutrient Agar (SNA) culture medium produced a higher number of this structure, developing 1,1x107 microconidia/ml and 0,7x107 microconidia/ml for F. guttiforme and F. ananatum, respectively. Using fluorescence microscopy, it was possible to observe that 78.5% of F. ananatum microconidia exhibit 1 septum, and only 8.5% of the microconidia of F. guttiform exhibit this characteristic, being a new recommendation for the taxonomy of the species. In atomic force microscopy, protuberances were found in the cell wall of F. guttiforme and absent in F. ananatum, and could be characterized as a new important morphological marker for taxonomy. In addition, the use of flow cytometry enabled the characterization of the distribution of core in the species, which establishes uninucleate microconidia and multinucleated macroconidia. The presence of several nuclei in macroconidia may be related to the amount of septa in its structure. The new phenotypic markers contribute to the taxonomy of pathogens and enable new strategies to identify the etiological agents of these diseases.