Influência do grau de sangue de doadoras Girolando na produção in vitro de embriões

• Ano de defesa: 2021
• Autor(a) principal: Moulin, Gabriel do Nascimento
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Mestrado em Ciências Veterinárias; Centro de Ciências Agrárias e Engenharias; UFES; Programa de Pós-Graduação em Ciências Veterinárias
• Palavras-chave em Português: Cruzamentos; Bovinos; PIVE; subject.br-rjbn; Ciências Agrárias
• Link de acesso: http://repositorio.ufes.br/handle/10/15455

The crossing between Bos taurus and Bos indicus, especially betweenthe Holstein x Gir breeds, are widely used in Brazil. However, information regarding the use of Girolando animals in an in vitro embryo production program (PIVE) is limited. The aim of this study was to evaluate the results of in vitro embryo production from donors Girolando ¼ (1/4 Holstein - ¾ Gir), ½ (½ Holstein - ½ Gir) and ¾ (¾ Holstein - ¼ Gir) donors, as well as analyzing the influence of the number of oocytes recovered, of the bull and the season on the PIVE indexes. Donors ½ (½ Holstein – ½ Gir) showed higher production of viable (19.7±0.6) and total (28.0±0.8) oocytes compared to ¼ (1/4 Holstein - ¾ Gir) oocytes 11.3 ± 1.0 viable and total 17.6 ± 1.4. Donors ½ were more efficient with 4.8 ± 0.2 produced compared to donors ¼ (2.6±0.3) and ¾ (1.6±0.3). Highest number of pregnancies (1.8±0.1) was found in donors ½ in relation to donors ¾ (0.6±0.2). Regarding the number of oocytes recovered, donors classified in the group of high oocyte production had a higher amount (28±3.0) and a percentage (72.4%) of viable oocytes compared to low production donors (13.3 ± 0.8; 68.9%), as well as a larger number of embryos produced (71.1±1.6 vs. 5.1±0.4). In the spring season, a higher percentage of oocytes was observed compared to winter (75% vs. 69,3%). Regarding the influence of the bull on the embryo and pregnancy rate, variations between 9.5 and 34.8% were found in the embryo rate, with no influence on the pregnancy rate. It is concluded that ½ donors are more efficient in embryo production than donors ¼ (1/4 Holstein - ¾ Gir), and ¾ (¾ Hostein - ¼ Gir). The winter negatively influences the percentage of oocytes recovered, and the bull has an influence on the percentage of embryos produced.