Análise da expressão temporal de genes relacionados ao metabolismo de Saccharomyces cerevisiae em resposta ao estresse por alta pressão hidrostática

• Ano de defesa: 2012
• Autor(a) principal: Silva, Lucas Ferreira da
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Mestrado em Biotecnologia; Centro de Ciências da Saúde; UFES; Programa de Pós-Graduação em Biotecnologia
• Palavras-chave em Português: Biotecnologia; 61
• Link de acesso: http://repositorio.ufes.br/handle/10/4454

Living organisms maintain a complex internal balance, which enables them to optimise their growth and metabolism. However, fluctuations in environmental conditions can affect this balance. The yeast Saccharomyces cerevisiae is a unicellular, eukaryote model organism. The biotechnological processes it is used for include the production of bread, cheese, alcohol, drugs and pharmaceuticals. From a physiological standpoint, all these processes affect the normal operation of the cell and it is extremely important to study the molecular mechanisms that provide protection against these stressful situations. One of the models used to study stress is high hydrostatic pressure (HHP), which is known to cause morphological and metabolic changes in yeast. Several studies have demonstrated that the expression pattern of genes from yeast submitted to high hydrostatic pressure shows a complex profile, including genes related to oxidative, osmotic, and heat protection.