Novas abordagens no diagnóstico não invasivo de câncer bucal utilizando espectroscopia ftir em amostras de sangue e saliva

• Ano de defesa: 2021
• Autor(a) principal: Thebit, Marcela Marçal
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Doutorado em Biotecnologia; Centro de Ciências da Saúde; UFES; Programa de Pós-Graduação em Biotecnologia
• Palavras-chave em Português: Biomarcador; ATR-FTIR; ácidos nucléicos; câncer oral; diagnóstico molecular; subject.br-rjbn; Biotecnologia
• Link de acesso: http://repositorio.ufes.br/handle/10/15185

Oral cancer is usually diagnosed through invasive methods, for although systemic changes can be easily detected, the identification of an effective systemic biomarker using a simple and affordable approach has yet to take place. Attenuated total reflection Fourier transform infrared (ATR-FTIR) appears as an alternative for the chemical and structural characterization of organic and inorganic materials, including liquid samples as serum, plasma and saliva and a possible bridge between molecular analysis and clinical practice. Objective: The aim of this study was to evaluate if ATR-FTIR spectroscopy can be applied as a tool in oral cancer screening through the analysis of plasma and salivary samples. Methodology: Plasma and saliva samples from oral cancer patients and controls were analyzed by ATR-FTIR spectroscopy. Experimental data were fed into the MATLAB 2018b software and them analyzed through two different routines in order to identify points of differentiations between the groups. Results: Biochemical analysis of the plasma samples through ATR-FTIR yielded 92% sensitivity and 100% specificity in differentiating between groups. The biggest difference was found between peaks that might be related to nucleic acids and nucleic acid phosphate. Pilot study with salivary samples reached similar accuracy Conclusion: We conclude that ATR-FTIR successfully discriminated between oral cancer patients and healthy subjects using plasma samples, with nucleic acids being found as major biomarkers.