Avaliação do potencial quimioprotetor de nanodispersão e extrato etanólico de Mikania glomerata Sprengel (asteraceae)

• Ano de defesa: 2016
• Autor(a) principal: Santana, Esdras Andrade
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Mestrado em Ciências Farmacêuticas; Centro de Ciências da Saúde; UFES; Programa de Pós-Graduação em Ciências Farmacêuticas
• Palavras-chave em Português: Nanodispersion; Antimutagenicity; Micronucleus assay; Mikania glomerata; Nanodispersão; Antimutagenicidade; Ensaio do micronúcleo; Asteraceae; Mutagênicos; Preparações farmacêuticas; Mutagênese; Farmacêuticos; Farmácia; 615
• Link de acesso: http://repositorio.ufes.br/handle/10/5304

Mikania glomerata is a medicinal plant having the coumarin as a chemical tracer, and due to bronchodilatory properties is widely used in traditional medicine in Brazil. The main objective of this study was to evaluate the potential of ethanol extract (EE) and nanodispersion (ND) of M. glomerata against cytotoxic and mutagenic effects induced by chemotherapy cyclophosphamide (CPA). This assessment was performed using micronucleus assay in bone marrow erythrocytes of Swiss albino mice (Mus musculus) in vivo through the simultaneous treatment protocol. The treatments using EE were performed with the 1.0; 0.5; 0.25; 0.008 e 0.004 mg.kg-1 concentrations of coumarin and ND in 0.008 e 0.004 mg.kg-1 concentrations of coumarin. The results showed that most of treatments reduced the cytotoxic damage caused by CPA. Only the treatment with EE 0.004 mg.kg-1 of coumarin did not show this effect. In the evaluation of antimutagenicity, the treatments with 1.0; 0.5; 0.25 mg.kg-1 concentrations of coumarin and ND (0.008 e 0.004 mg.kg-1 of coumarin), demonstrated to be effective in reducing the micronucleus frequency, which means they are antimutagenic agents. The treatment using ND (0.008 mg.kg-1 of coumarin) showed a 60.38% damage reduction, indicating greater effectiveness among the treated groups. Overall, treatments with nanodispersions were more efficient in chemoprotective action, although its low coumarin content in the experimental conditions. The results reinforce the importance of using nanotechnology to transport of natural compounds and the potential of compounds extraction technology through nanodispersions.