Determinação de estanho em tecidos de ratas por ICP-MS

• Ano de defesa: 2015
• Autor(a) principal: Brandão, Poliane Aparecida Alves
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Mestrado em Química; Centro de Ciências Exatas; UFES; Programa de Pós-Graduação em Química
• Palavras-chave em Português: Amostras biológicas; ICP-MS; Tin; Estanho; Espectrometria de massa; Química; 54
• Link de acesso: http://repositorio.ufes.br/handle/10/1497

The organotin compounds, especially tributyltin (TBT), are environmental contaminants are mainly used in antifouling paints for ships. The organotin compounds, especially tributyl (TBT), environmental contaminants are mainly used in antifouling paints for ships. They may bioaccumulate and can be found in mammals, including humans. The main source of exposure is ingestion of contaminated food. This study aimed possessed the determination of tin in rats exposed tissue chronically TBT using mass spectrometry with inductively coupled plasma (ICP-MS). Adult Wistar rats were on trial, and divided in two groups: the group exposed to 100 ng TBT kg-1 day-1 for 15 days and the reference group receiving only the vehicle during the same period of exposure. At the end of exposure, the animals were sacrificed and plasma, heart, kidney, lung, liver and ovarian were collected for analysis. The samples were dried for 72 hours and about 100 mg of each sample were subjected to acid digestion microwave. The determination of tin was carried out by ICP-MS after acid digestion microwave assisted by a sample with approximately 100 mg. The limit of detection (LOD) was calculated 4.3 ng L-1, which allows the determination of tin in tissue samples from animals used for experimentation. Accuracy was assessed by analyzing the certificate urine reference material Seronorm Urine (54.6 ± 2.7 ug L-1), resulting in 50.1 ± 3.8 ug L-1. After validation, the tin concentration was determined in plasma, heart, kidney, lung, liver and ovarian samples in the TBT group and in the control group. There was a statistically significant difference between the two groups for all samples. Differences between groups were most prominent in samples of liver and kidney. Furthermore, this study showed that the presence of tin in rat’s body is distributed in the tissues, resulting in morphological and physiological changes already founded in ovary, heart and liver