Vírus entéricos e indicadores bacteriológicos de poluição fecal em amostras de água na região da Ilha das Caieiras, na Baía de Vitória, ES

• Ano de defesa: 2012
• Autor(a) principal: Loss, Susanne Mariani
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Mestrado em Engenharia Ambiental; Centro Tecnológico; UFES; Programa de Pós-Graduação em Engenharia Ambiental
• Palavras-chave em Português: Estuary; Enteric Virus; Fecal coliforms; Enterococci; Water; Vírus entéricos; Estuários; Microbiologia - Aspectos ambientais; Água poluída por vírus; Coliformes fecais; Enterococcus; Engenharia Sanitária; 628
• Link de acesso: http://repositorio.ufes.br/handle/10/6128

The Ilha das Caieiras is located in one of the poorest regions in Vitória / ES, the Grande São Pedro. This region is densely populated and is known as a pocket of poverty in the capital, with a population surviving mainly on the collection and marketing of seafood mangrove located in their surroundings. Previous studies have shown that the region of this estuary water is contaminated with pathogenic microorganisms from mainly domestic sewage released without prior treatment. The aim of this study was to evaluate the presence of enteric viruses (adenovirus - AdV, rotavirus - RV and norovirus GII - NoV) and bacterial indicators of fecal pollution (fecal coliform and enterococci) in water samples from four sites of this important Ilha das Caieiras’ estuary. Monitoring occurred from January 2011 to July 2012 (19 months) and used molecular techniques Qualitative PCR and Real Time PCR (qPCR) for detection of enteric viruses, and membrane filter for analysis of bacteria, and assessment of physico-chemical parameters of the water. The microbiological analysis of water studied sites (P1, P2, P3 and P4) showed the presence of fecal coliform, with geometric means of 1.66 x102 , 1.31 x102 , 2.29 x103 and 3.13 x102 CFU / 100 mL water, respectively. For enterococci, the averages were 6.30 x101 CFU / 100 mL in P1, 5.56 x101 CFU / 100 mL at P2, 1.89 x103 CFU / 100 mL at P3 and 1.62 x103 CFU / 100 mL at P4. Enteric viruses were detected in the four monitoring sites by both molecular techniques used, with maximum values of 2,00x103 , 5,24x104 and 1,50x104 GC / 100 mL for AdV, NoV and RV, respectively, quantified by qPCR. The frequencies of virus detection in samples of estuarine water ranged from 21 - 27% for AdV, 42 - 53% for RV and 10 - 42% for NoV GII. In this study we found that the estuary of the Ilha das Caieiras presents contaminated, possibly due to sewage releases fresh and / or leachate from rural areas. The anthropogenic mangrove on this is clear and worrisome because environmental quality is reflected in this environment, the health of the population and the economy of the region.