Avaliação morfofisiológica, fitoquímica e mutagênica de Passiflora edulis Sims f. flavicarpa Deg exposta a diferentes concentações de alumínio

• Ano de defesa: 2012
• Autor(a) principal: Perdigão, Tatiane Lemos
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Mestrado em Biologia Vegetal; UFES; Programa de Pós-Graduação em Biologia Vegetal
• Palavras-chave em Português: Soil aluminium; Secondary metabolics; Flavonoids; Mutagenicity; Micronucleus; Metabólitos secundários; Flavonoides; Micronúcleo; Solos - Teor de alumínio; Testes de mutagenicidade; Nucleolo; Agronomia; 57
• Link de acesso: http://repositorio.ufes.br/handle/10/5738

The influence of aluminum (Al) in two cultivars of Passiflora edulis is presented in two chapters: first a discussion on the experiment with the cultivar (cv.) FB 100 and second with the cultivar (cv.) FB 200. The study with cv. FB100 in greenhouse aimed to verify morphophysiological changes after 15 days of treatment with Al. For cv. FB 200 plants was evaluated the relationship between the mutagenic and the concentration flavonoids produced by them, when grown in soils with different concentrations aluminum. Analyses of fresh and dry weight organs, stem length, leaf area, nutritional analysis and verification of Al in leaves and roots were studied on cv.FB100. For the leaves of cv. FB100 and cv. FB200 phytochemical screening of extracts were used staining and precipitation reactions for identification of the main chemical classes present. The concentration of the flavonoids was determined by spectrometric measurement. To mutagenicity test was found the frequency of micronucleus in 2000 polychromatic erythrocytes using the cv. FB200 leaves extract. All cv. FB100 morphological parameters analyzed did not differ from controls. However a higher Al concentration was presented in the treatment of 5.32 mM roots. All treatments showed the presence of alkaloids, flavonoids and steroids in leaves. The extract hexane/dichloromethane at a higher flavonoid concentration, has been observed in treating 0.33 mM Al. The cv. FB200 results showed higher foliar Al concentration in the field with 0.33 mM Al (T2), and saponins, steroids and flavonoids were the major groups found. The T2 treatment had the highest flavonoids concentration and higher mutagenic potential. These results indicate that Al did not promote significant changes in cv. FB 100 morphophysiological parameters. In P.edulis cv. FB 200 saponins, flavonoids and steroids occur regardless of the Al available and possibly the largest concentration of flavonoid mutagenicity can be induced in cells of mouse bone marrow