Eficácia antinociceptiva e anti-inflamatória de lectina de abelmoschus esculentus no modelo de hipernocicepção inflamatória induzida por zymosan na articulação temporomandibular de ratos

• Ano de defesa: 2015
• Autor(a) principal: Freitas, Raul Sousa
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Abelmoschus esculentus; articulação temporomandibular; hipernocicepção; HO-1; lectina
• Link de acesso: http://www.repositorio.ufc.br/handle/riufc/30330

Abelmoschus esculentus (Okra), an African plant cultivated in northeastern Brazil, is used for different medicinal purposes. Here we investigated Abelmoschus esculentus lectin (AEL) efficacy in zymosan-induced temporomandibular joint (TMJ) inflammatory hypernociception in rats. Rats were pretreated with AEL (0.01, 0.1 or 1 mg/kg) before zymosan (Zy) injection in the TMJ. Further, rats were pretreated with ZnPP-IX (3 mg/kg), a specific HO-1 inhibitor, or aminoguanidine (30mg/kg), a selective iNOS inhibitor, before AEL. Von Frey test was used to evaluate hypernociception. 6 h after Zy synovial fluid was collected for leukocyte counting and myeloperoxidase (MPO) activity; and TMJ tissue for histopathological analysis (H&E) and immunohistochemistry for TNF-α, IL-1β, and HO-1. Vascular permeability was evaluated by Evans Blue extravasation. Also, TMJ tissue and trigeminal ganglion were removed for TNF-α and IL-1β dosage (ELISA). AEL increased nociceptive threshold. AEL reduced cell influx, MPO activity, cell influx in synovial membrane, and Evans Blue extravasation. AEL increased HO-1 expression while decreased both TNF-α and IL-1β expression. AEL effects were not observed in the presence of ZnPP-IX. AEL reduced TNF-α and IL-1β levels in both TMJ tissue and trigeminal ganglion. AEL efficacy in zymosan-induced TMJ inflammatory hypernociception in rats depends on TNF-α/IL-1β inhibition and HO-1 pathway integrity.