Efeito modulador de proteínas do látex de Calotropis procera no processo inflamatório agudo em animais infectados com Salmonella

• Ano de defesa: 2020
• Autor(a) principal: Sousa, Brandon Ferraz e
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Citocinas; Coagulação; Hemostasia; Óxido nítrico; Peritônio
• Link de acesso: http://www.repositorio.ufc.br/handle/riufc/52642

Calotropis procera is a plant whose parts have been used in alternative medicine, aiding in treatment of various pathologies, such as: joint pain, asthma, malaria, eczema, leprosy, bleeding, skin burns and inflammation. The process of dialysis and centrifugation of the latex of C. procera results in protein fractions with differentiated effects over the cell immunity. The protein fraction LP and its subfractions (LPPI, LPPII and LPPIII), administered by different routes in animal models, induce different effects over cell mediated immunity in the organism. The current study aimed to evaluate the modulatory capacity of protein fractions LPPII and LPPIII from Calotropis procera latex in a model of inflammation induced by lethal infection by Salmonella Typhimurium. The latex protein fraction (LP) obtained was fractionated by ion-exchange chromatography to obtain three fractions: LPPI, LPPII and LPPIII. Swiss mice were treated with LPPII and LPPIII (5 and 10 mg/kg), by intraperitoneal route, and then were challenged with S. Typhimurium in lethal injections (106 UFC/ml). All treatments protected the animals in different degrees, but LPPIII the dose of 5 mg/kg exhibited a bigger survival rate and, consequently, were used to evaluate the following parameters: 1- bacterial enumeration in blood, peritoneal fluid, spleen and liver; 2- histopathological analysis; 3- total and differential count of leukocytes in peripheral blood and peritoneal cavity; 4- effect over the total plasma coagulation; 5- modulation of cytokines and nitric oxide. The fraction LPPIII (5 mg/kg) was capable of protection 70 % of animals against lethal infection. This protective effect was due to the control of the onset inflammation, through modulation of IL-1β, IL-10 and NO release, inducing an inflammatory stimulus prior to the infection, mainly by increasing the neutrophilic infiltration in the infection locus. It was also observed the maintenance of hemostasis of the coagulation pathways. These results demonstrate the biotechnological potential present in the latex of Calotropis procera, it also corroborates and expands upon the knowledge regarding the mechanism of protection against Salmonella Typhimurium infection reported for LP.