Caracterização, resfriamento e criopreservação de sêmen de tilápia-do-nilo cultivada no estado do Ceará

• Ano de defesa: 2013
• Autor(a) principal: Teixeira, Erivânia Gomes
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Recursos pesqueiros e engenharia de pesca; Oreochromis niloticus; Indução anestésica; Caracterização espermática; Conservação de gametas; Anesthetic induction; Sperm characterization; Conservation of gametes; Tilápia (peixe) - Espermatozóides; Sêmen; Criopreservação
• Link de acesso: http://www.repositorio.ufc.br/handle/riufc/18707

The present study deals with anesthetic induction, characterization of sperm fluid, cooling and freezing of sperm of the Nile tilapia Oreochromis niloticus. In the first chapter the natural anesthetics menthol and eugenol were tested in adult specimens aiming to determine the ideal concentration for handling the Nile tilapia. In the second chapter the sperm fluid of the Nile tilapia was characterized according to the following parameters: collected volume, which showed the feasibility of collect in sufficient volume without the need of sacrificing the fish; sperm concentration, which showed significant variation between specimens; pH and osmolarity, which confirm the information of the literature; sperm motility time, which proved that the spermatozoa of tilapia are differentiated concerning accumulated energy when compared to other freshwater species; and sperm head morphometry, characterized by size (length, width, perimeter and area) and head shape (ellipticity, rugosity, elongation and regularity). In the third chapter we evaluated the feasibility of preservation of tilapia sperm using different extenders and cooled at 4°C. The sperm motility rate was evaluated with the aid of the Computer-assisted Sperm Analyzer (CASA) System. In this, the ACP-104, media based on powdered coconut water, showed best sperm motility rate for up to 72 hours after storage. In the fourth chapter was evaluated the effects of different cryoprotectants on the post-thaw Nile tilapia sperm, using the software Sperm Class Analyzer (SCA). The results of the sperm motility rate and sperm velocity parameters revealed that ACP-104 in combination with dimethylsulfoxide is a promising cryodiluent for Nile tilapia sperm in long-term conservation.