Síntese de Nanocápsulas de Amido e Hidroxietilamido via Miniemulsão Inversa para o Carreamento de Fármacos

• Ano de defesa: 2019
• Autor(a) principal: Santos, Sarah Brenda Ferreira dos
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Nanocápsulas poliméricas; Amido; Síntese via miniemulsão; Staphylococcus aureus
• Link de acesso: http://www.repositorio.ufc.br/handle/riufc/68462

The resistance of microoganisms has became a challenge considering the conventional pathologies treatment and the overusage of antibiotics, providing several adverse side effects and clinical dosage failure. Thus, the development of “smart” drug delivery systems emerges as a great strategy, principally aiming to minimize the side effects, and also to improve the therapeutic action of medications. The synthesis of starch-based nanocapsules via inverse miniemulsion is a potential alternative for conventional drug formulations, since their core-shell morphology enables an efficient encapsulation of biologic active substances, such as fluoxetine hydrochloride and troxerrutin. were characterized regarding structural, thermal and composition analysis by FT-IR, DSC and HPLC, respectively. The polysaccharides were characterized by FT-IR and GPC. The nanocarriers systems synthesized in this work were characterized by DLS in order to verify the average hydrodynamic diameter, which showed values between 251 and 360 nm, in addition, the values of zeta potential were higher than -30 mV, showing great colloidal stability for all systems . From FT-IR analysis, it was possible to observe bands characteristic of the formation of polyurethane linkages, due to polyaddition reaction. The morphology of the nanocapsules was evaluated by SEM and TEM, where it was possible to observe the formation of the core-shell structure of the systems. In addition, analyzes of solid content (SC%) were carried out, which presented concentration of g of nanocapsules per g of solution in percentage around 0.13% - 0.34%. The Efficiency of Encapsulation (EE) as well as the Carrying Capacity (CC) presented values >90% for EE and >2.0% for CC, demonstrating an ideal synthesis strategy for application in pharmaceutical formulations. Finally, the nanocapsules had their antibacterial potential tested against Staphylococcus aureus microorganisms (ATCC® 6538P ™), and two clinical strains of methicillin-resistant Staphylococcus aureus, in which free troxerrutin showed no activity. However, the control nanocapsules and nanocapsules with fluxetine presented antibacterial activity with MIC values in the range of 190-95 μg/ml against all isolated microorganisms.