Evidências de alterações comportamentais e neuroinflamatórias a longo prazo em camundongos submetidos ao modelo de depressão por privação materna sozinha ou associada a desafio imune com lipopolissacarídeo

• Ano de defesa: 2018
• Autor(a) principal: Cunha, Natássia Lopes
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Depressão; Inflamação; Privação Materna; Envelhecimento
• Link de acesso: http://www.repositorio.ufc.br/handle/riufc/36798

One of the triggering factors for depression is exposure to early adversity (EA) in life. These early experiences are also related to suicide in adulthood. One of the animal models with great translational validity for the study of depression is the maternal deprivation model, which simulates the exposure to EAs. In this context, the objective of this study was to study the changes in adult age (PND 60) and senescence (PND 180), triggered by maternal deprivation, associated or not to immune challenge with LPS (0.5mg / kg i, p.) In both the ages. Male C57BL / 6 mice were used. For this, the animals were deprived of the mother for 13 days, 1h per day and were submitted to the following behavioral tests: sucrose preference and forced swimming test for evaluation of type-depression behavior, open field and light/dark for determination of anxiety, recognition of the new object and Y-maze to evaluate memory. In addition, oxidative changes were analyzed from the reduced glutathione (GSH), lipid peroxidation (MDA) and myeloperoxidase (MPO), IL-4, IL-6 and TNF-α immune changes in serum and the hippocampus and microglial activation. Our results showed that maternal deprivation was able to induce anxious, noticeable behavior in the open field test and light-dark test and type-depressive behavior evidenced in the forced swimming test and sucrose preference. PS was able to exacerbate the effects of maternal deprivation, both in adulthood and in senescence. Maternal deprivation associated with LPS significantly increased MDA and MPO levels and reduced GSH levels. At the cytokine levels, LPS brought significant increases and the association with maternal deprivation showed an increase in IL-6 and TNF-α levels. In addition, a significant increase in microglial activation was observed in all areas of the hippocampus, especially with the association of maternal deprivation with LPS. In general, we observed that exposure to LPS at different ages of life (adult and senescence) is capable of modifying the course of the changes caused by EA exposure due to maternal deprivation, being the knowledge of the mechanisms underlying these changes of great importance for the establishment of prevention strategies.