Aspectos pró-inflamatórios do óleo essencial de Pilocarpus spicatus St.Hill

• Ano de defesa: 1993
• Autor(a) principal: Silva, José Clielder Rebouças da
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Pilocarpus; Óleos Voláteis; carragenina
• Link de acesso: http://www.repositorio.ufc.br/handle/riufc/65082

The essential oil of Pilocarpus spicatus (EOPS) was evaluated for its proinflammatory activity in rats and mice. In doses ranging ffom 2,5 to 5mg/site EOPS induced a dose-dependent paw edema. The effect was apparent after lhr, peaked at 3hr and after 24hr it still persisted at 50% levei. The edematogenic potential of EOPS is almost comparable to that of carrageenan, a polysaccharide obtained ffom algae. However, at 24hr following intraplantar injection the carrageenan response was almost disappeared when compared to EOPS. Also histological studies revealed a progressive lesion with increased neutrophil infiltration in the rat hind paw after intraplantar injection of EOPS (1,5 and 24hr). The intrathoracic administration of EOPS (lOmg/site) induced pleurisy characterised by exudate formation and leukocyte migration in a manner similar to carrageenan with potent effect at 5hr. Granuloma induced by subcutaneous implants of cotton pellets was enhanced by EOPS as well as carrageenan (p<0,01). Also EOPS (5 and lOmg/site) have caused an increase in capillary permeability upon intraperitoneal injection as evidenced by extravasation ofEvans blue dye. The increases were 64 and 84%, respectively, as compared to carrageenan. In order to identify the possible inflammatory mediators of EOPS-induced paw edema, the effect of various drugs was analysed. Among the drugs tested, dexamethasone (0,5mg/kg,i.p.) was the most effective and was able to inhibit paw edema at all time periods studied. The Hj antagonist chlorpheniramine (10mg/kg,i.p.) was ineffective, while the antiserotonergic methisergide (lmg/kg.p.o.) was found to be effective in the first phase reaction (1 and 2hr). Cicloxigenase inhibitor phenylbutazone (lOOmg/kg, p.o.) and the lipoxygenase inhibitor EP 10161(20mg/kg,i.p.) were effective in preventing peak edema response (3hr). The mast cell degranulator 48/80 significantly inhibited the lhr edema reaction as well as at the 5hr. These observations suggest that EOPS has pro-inflammatory activity which is mediated by serotonin and eicosanoids.