Estudo hepático de um modelo murino dietético para síndrome metabólica: perfil morfológico, funcional e balanço redox.

• Ano de defesa: 2009
• Autor(a) principal: Guedes, Glaucevane da Silva
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Alagoas; BR; Nutrição; Programa de Pós-Graduação em Nutrição; UFAL
• Palavras-chave em Português: Metabolic syndrome; Liver functions; Redox balance; Novel diet-induced mice model; Síndrome metabólica; Funções hepáticas; Balanço redox; Novo modelo murino dietético; CNPQ::CIENCIAS DA SAUDE::NUTRICAO
• Link de acesso: http://repositorio.ufal.br/handle/riufal/635

Changes in lifestyle of people has been dynamically observed in recent decades, emphasis is given to dietary issues. In this case, as directly responsible for chronic diseases, along with other aspects of lifestyle such as inactivity, smoking, quality of life. In the context of chronic diseases of high incidence and prevalence, the metabolic syndrome has gained prominence in clinical and experimental research in the search for early diagnostic methods for its various components such as the vascular, biochemical and liver, all also associated with diet. In this context, there was the inclusion of non-alcoholic fatty liver disease as liver component of the metabolic syndrome, which is the central object of this work. Objective: To evaluate the profile morphological, functional and redox balance in the liver of a novel induced-diet mice model for metabolic syndrome. Methods: Eleven isogenic male C57BL/6J mice were randomly divided into control (CT) and hypercaloric (HC) groups and fed with chow and hypercaloric diet, respectively, for 26 weeks. We did ratings serum markers of liver function (ALT, AST, ALP, γ-GT, LDH, albumin and total protein), analysis of local redox status (SOD, CAT and lipid peroxidation) and histological study of tissue. Results: There were significant elevations of the statistical point of view in the main markers of liver function in HC animals. This increase was approximately 1.5 times for ALT, 2.5 for AST and 7.4 for ALP. The activity of γ-GT followed the pattern of significant increase in the HC group, despite its low activity. There re no differences in the concentrations of albumin and total protein. On the local redox state, the activity of SOD was not different between groups, the catalase activity was significantly decreased in HC group and there re high levels of peroxidation in this same group. The histological findings corroborate these results, which corresponds to advanced liver disease in animals HC (default cirrhotic). Conclusion: The hepatic component in the novel induced-diet mice model for metabolic syndrome shows changes in your profile functional, redox balance and morphological. Keywords: Metabolic Syndrome, Liver Functions, Redox Balance, Novel Diet-induced Mice Model.