Investigação do efeito cardioprotetor do extrato hidroalcoólico de própolis vermelha em ratos infartados por modelo simpatomimético

• Ano de defesa: 2015
• Autor(a) principal: Clementino Neto, José
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Alagoas; Brasil; Programa de Pós-Graduação em Ciências Farmacêuticas; UFAL
• Palavras-chave em Português: Infarto do miocárdio - Ratos; Isoproterenol - Utilização; Própolis Vermelha – Efeito cardioprotetor; Myocardial infarction - Rats; Isoproterenol - Use; Red propolis - Cardioprotective effect; CNPQ::CIENCIAS DA SAUDE::FARMACIA
• Link de acesso: http://www.repositorio.ufal.br/handle/riufal/6704

Propolis is a natural resinous substance produced by bees of species Apis mellifera, through a variety of plant sources and various parts of the plants. Its chemical composition is complex and diverse. The red propolis is a type of propolis found in the mangroves of Brazilian northeastern. Some biological activities are associated with red propolis, among them, antioxidant, antimicrobial and anti-inflammatory activities. The present study was designed to evaluate the cardioprotective effect of hydroalcoholic extract of red propolis from Alagoas (HAERP) against isoproterenol-induced myocardial infarction in rats. For chemical characterization of HAERP was performed high performance liquid chromatography coupled to ultraviolet detector (HPLC-UV). Male Wistar rats (200-300g) were divided into 5 groups (n = 5), (Control = 0.3 mL Saline p.o. for 30 days), (ISO = infarction by isoproterenol 85 mg/kg s.c. on 2 consecutive days), (HAERP 50, 75 and 150 mg/kg, respectively, orally for 30 days and underwent infarction with isoproterenol 85mg/kg s.c in 29 and 30 days of treatment). On the 31st day the animals were euthanized, serum was collected and incubated in biochemical kits for the evaluation of creatine kinase total fraction (CK-NAC) and MB fraction (CK-MB), lactate dehydrogenase (LDH), aspartate transaminase (AST), alanine transaminase (ALT), cholesterol total (COL) and triglycerides (TRI) markers of tissue damage. For morphometric analysis, were measured the weight cardiac, the thickness ventricular of left and right, its variations and relative increases, and reason cardiac weight by body weight (WC, TLV, TRV and WC/WB). For postmortem analysis the hearts were sectioned yet submitted to colorimetric test of triphenyltetrazolium chloride 1% (TTC1%). The results were expressed as mean ± SEM, and analyzed statistically by ANOVA one-way followed by Newman-keuls. Considered significant when *p<0,05, **p<0,01, ***p<0,001 vs. control; +p<0,05, ++p<0,01, +++p<0,001 vs. ISO; •p<0,05, ••p<0,01, •••p<0,001 vs. HAERP 50 mg/kg + ISO. 14 different substances were identified in HAERP, among them, some flavonoids and phenolic acids. TLV (G1= 3.8 ± 0.4; G2= 4,9 ± 0.1***; G3= 4,6 ± 0,2***; G4= 3,8 ± 0,2•••/+++; G5= 3,8 ± 0,2•••/+++ mm), WC/WB (4,20 ± 0,12; 5,43 ± 0,10***; 4,71 ± 0,20+++; 4,77 ± 0,20+++; 4,78 ± 0,20+++), CK-NAC (471,2 ± 31,2; 1035 ± 134,7***; 697,7 ± 47,6++; 578,7 ± 57,14+++ ; 397,9 ± 34,58+++/• U/L), CK-MB (414,4 ± 18,5; 740,8 ± 77,3***; 505,6 ± 43,5+++; 496,0 ± 32,9++; 376,8 ± 6,3+++ U/L). The results indicate that the treatment with HAERP was able to prevent hypertrophy concentric cardiac, it is observed that there is a decrease in TLV and WC/WB. In the biochemical analysis observed a reduction in counting enzyme CK-NAC and CK-MB fraction after treatment with HAERP in three doses. There was no significant change in LDH, COL and TRI counting, in three doses, when compared to control. The colorimetric test revealed there wasn´t development necrotic areas in the heart. The results indicate that treatment with HAERP was able to promote protective activity in myocardial infarction.