Associação entre a Na+, K+-ATPase e a fertilidade de amostras seminais descongeladas de touros Angus

Detalhes bibliográficos
Ano de defesa: 2019
Autor(a) principal: Marques, Juliana Carla Cavalcanti
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Alagoas
Brasil
Programa de Pós-Graduação em Inovação e Tecnologia Integrada a Medicina Veterinária para o Desenvolvimento Regional
UFAL
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: http://www.repositorio.ufal.br/handle/riufal/7001
Resumo: Currently, one of the challenges on bovine reproduction is to efficiently identify high fertility bulls, since traditional andrological evaluation may not precisely differ the fertilizing potential of a sire, leading to reduced pregnancy rates and economic losses. Therefore, the Na+, K+-ATPase has been considered a potential fertility marker, due to its sperm specific properties and reports o correlation with Holstein bull fertility. However, specificities on reproductive management of Brazilian beef herds associated to the lack of information regarding Na+, K+-ATPase in beef bulls, the present study was performed aiming to determine association between Na+, K+- ATPase activity in thawed Angus bull sperm scored as being a high fertility (HF) or normal fertility (NF) after fixed time artificial insemination (FTAI). Samples from three different commercial batches of bulls of HF (n=4) or NF (n=4) were used. Sperm kinematics was evaluated with CASA system, sperm viability was assessed by fluorescent microscopy, and expression of Na+, K+-ATPase on the sperm surface by flow citometry, immediately post-thaw (0h) and after 2h of incubation (37 °C). Na+, K+-ATPase activity was determined measuring the releasing of inorganic phosphate (iP) in the samples treated or not with a specific inhibitor (ouabain, 4×10-4 M), normalized by total protein of each sample. Within the same incubation time, there was no difference (P > 0.05) on sperm viability, kinematic parameters, and the expression of Na+, K+-ATPase between HF and NF bulls. Kinematic parameters of LIN and VCL were not influenced (P>0.05) by incubation time in samples from HF and NF, respectively. There was a tendency (P=0.06) of higher Na+, K+-ATPase enzymatic activity in HF bull samples. In conclusion, differences on kinematic and sperm viability are not detected between HF and NF Angus bulls immediately postthaw. Different responses on velocity parameters post-incubation were detected in HF and NF bulls that might be related to Na+, K+-ATPase activity, without influence of Na+, K+-ATPase expression.