Metabolismo de lipídeos em inseto coleóptero: digestão e transporte de ácidos graxos

• Ano de defesa: 2018
• Autor(a) principal: Freire, Camilla Camerino Santana Davino
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Alagoas; Brasil; Programa de Pós-Graduação Multicêntrico em Bioquímica e Biologia Molecular; UFAL
• Palavras-chave em Português: RNA de interferência (RNAi); Reação em cadeia da polimerase quantitativa (qPCR); Lipídeos; Tribolium castaneum; Coleoptera; Fatty Acid Transport Protein; Interference RNA; Quantitative polymerase chain reaction; Lipids; CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR
• Link de acesso: http://www.repositorio.ufal.br/handle/riufal/3421

Coleoptera is an order of insects well known as beetles. Most coleopteran species are phytophagous insects and for this reason are essential to crop and storage pests such as the Tribolium castaneum. Lipid metabolism is vital for the biological functions of insects, playing a role in the generation of metabolic energy and other cellular processes. Fatty acid transport proteins (FATPs) play a crucial role in the transport of extracellular fatty acids to cells, have a conserved sequence between species and are involved in the synthesis of hormones and pheromones. Recent studies show that silencing the gene for FATPs through interfering RNAi techniques (RNAi) in insects affects fatty acid uptake and pheromone synthesis. This work aims to characterize proteins homologous to FATPs present in the genome of Tribolium castaneum, to evaluate the gene expression in tissues, developmental stages and insects treated with Orlistat and to evaluate the effect of FATP silencing on energy metabolism. Bioinformatics analyzes were performed with the amino acid sequences, and real-time PCR evaluated the gene expression. The effects of the drug Orlistat were evaluated through qPCR and analysis of nutritional index. The search for sequences in the T. castaneum genome revealed two sequences of proteins homologous to FATPs and bioinformatic analysis was performed. The study of the gene expression of FATPs by qPCR demonstrated more significant expression of the two genes in the fat body of larvae and many expressions in all stages of development of the insect, with higher expression in the pupa stage. The effects of Orlistat on the expression of FATPs evidenced the influence of diet composition on the regulation of the gene expression of these proteins. Gene silencing of TcasFATP was achieved, but no direct effects on the energetic dynamics of the larvae were observed since triacylglycerol levels, and β-oxidation rates were not affected. Thus, more detailed studies with the use of gene silencing will be necessary to characterize FATPs better and elucidate their role in insect energy metabolism.